急性白血病患者外周血HMGA1基因的表达  

Detection of HMGA1 Expression in Peripheral Blood of Acute Leukemia Patients with SYBR Green I Real-time Quantitative RT-PCR

作　　者：郝明巨[1] 李稳[1] 刘敏[2] 叶翎[3] 

机构地区：[1]山东省中医院检验科,邮政编码济南250011 [2]济南市皮肤病医院检验科 [3]济宁市第一人民医院血液科

出　　处：《微循环学杂志》2009年第4期38-40,共3页Chinese Journal of Microcirculation

摘　　要：目的:检测急性白血病(AL)患者外周血高迁移率蛋白A1(HMGA1)基因表达水平。方法:采用SYBR Green I RQ-PCR检测30例AL,包括25例AML(M2～M7)和5例ALL(AL组),25例非白血病患者(对照组)外周血HMGA1 mRNA表达水平。结果:AL组HMGA1 mRNA表达明显高于对照组,ALL和AML间HMGA1 mRNA表达水平无显著差异;AML中M7的HMGA1 mRNA表达水平高于M2～M5。结论:HMGA1 mRNA在AL患者外周血高表达,其中M7水平最高,可作为白血病基因治疗的新靶点及M7的鉴别诊断指标。Objective:To investigate high-mobility group A1 (HMGA1)mRNA expression levels in peripheral blood(PB) of acute leukemia patients (ALs).Method: To adopt SYBR Green I real-time quantitative polymerase chain reaction(RQ-PCR) for detecting HMGA1 in PB of 30 ALs including 25 AMLs(M2～M7)and 5 ALLs,25 non-leukemia controls. Results: The expression of HMGA1 mRNA in 30 patients with acute leukemia were statistically higher than those in 25 non-leukemic controls;the expression levels between ALL and AML had no significant difference,the expression of HMGA1 mRNA in megakaryocytic leukemia was the highest in all groups.Conclusion: HMGA1 expression levels in ALs were strikingly higher than that in non-leukemias,and were the highest in M7.HMGA1 can be the new target of gene therapy and the differential diagnosis index of M7.

关 键 词：急性白血病患者 A1基因 HMG 外周血 高迁移率族蛋白 Protein 染色质结构 非组蛋白 

分 类 号：R733.710.7[医药卫生—肿瘤] R589.2[医药卫生—临床医学]