产漆酶木腐真菌的筛选、鉴定及部分酶学性质  

Screening and Identification of Laccase-producing Wood-rot Fungi and Characterization of Its Enzyme

作　　者：王剑锋[1] 李江[1] 饶军[1] 王璋[2] 

机构地区：[1]东华理工大学生物系,江西抚州344000 [2]中国食品发酵工业研究院,北京100027

出　　处：《食品与发酵工业》2009年第10期44-48,共5页Food and Fermentation Industries

摘　　要：综合菌体培养方式和培养基组成对菌株产漆酶性能的影响,对68株木腐菌进行了筛选,获得了1株适合液态发酵产漆酶的漏斗多孔菌A08,酶学性质研究表明,漆酶反应最适温度为60-65℃,最适pH为5.5,60℃时酶活半衰期大于45min,20℃酶活性在pH3.0-9.0稳定,漆酶活性受Cl-、Ca2+、Fe2+抑制,Mn2+激活,K+、Mg2+、Zn2+、Cu2+等对酶活性没有显著影响。Based on the effects of culture method and medium composition upon productivity of laccase from Wood-rot Fungi, a strain with relative higher lacease-producing ability in liquid culture was screened from 68 strains of wood-rot fungi, and identified as Polyporus arcularius. Studying on enzymatic properties revealed that the optimum temperature and pH of the lacease reacted with guaiaeol were 60 - 65 ℃ and pH 5.5, respectively. The half-life of the laccase was more than 45 min at 60℃. The enzyme activity was stable within pH range of 3.0 -9.0 under 20℃ , was strongly inhibited by Fe^2＋ , and moderately by Cl^- , Ca^2＋ , while enhanced by Mn^2＋ Wheres other ions like K^＋ , Mg^2＋ , Zn^2＋ , Cu^2＋ had no evident effects on it within the range of experimental concentration.

关 键 词：漆酶 漏斗多孔菌 酶学性质 培养方式 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]