高效液相法同时测定混合环糊精中的α,β,γ-环糊精的含量  

HPLC For Simultaneously Determination of a Mixed-cyclodextrin of α,β,γ-Cyclodextrin

作　　者：毛勇[1] 邓媛[1] 李皎[1] 汪大敏[1] 杨国武[1] 

机构地区：[1]陕西省微生物研究所,陕西西安710043

出　　处：《食品与发酵工业》2009年第10期138-140,共3页Food and Fermentation Industries

摘　　要：建立了同时测定环糊精中α-环糊精、β-环糊精、γ-环糊精3种成分含量的高效液相色谱法。方法采用色谱柱为SpherrigeL C6H5(5μm,300mm×3.9mm),流动相V(甲醇)∶V(水)=5∶95进行洗脱,流速为1.0mL/min,检测器为示差折光检测器,柱温40℃,进样量20μL。α-环糊精、β-环糊精、γ-环糊精的检出限分别为1.03,2.53,1.50μg/g,线性范围分别为2.0-207.9、11.5-230、3.9-194.2μg/mL,精密度相对标准偏差(n=6)分别为0.12%,1.13%,0.43%,加样回收率(n=6)平均分别为98.5%、98.0%、98.1%。该方法简便、灵敏、准确,可用于混合环糊精中α-,β-,γ-CD的含量测定和质量控制。An HPLC method for determination of three kinds of cyclodextrins, α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin was developed. The system was ： Spherrigel C6H5 column（5μm, 300 mm × 3.9 mm） , methanol-water （5：95, v/v）as the solvent, the flow rate of 1.0 mL/min（column temperature 40℃ ）with the refractive index detector. The results were obtained that the detection limit ofα-CD, β-CD, γ-CD were 1.03 μg/g,2.53 μg/g,1. 50 μg/ g, linear range of the three kinds of cyclodextrin were 2.0 - 207.9 μg, 11.5 - 230 μg, 3.9 - 194.2 μg respectively, relative standard deviation （n = 6） were 0. 12% , 1.13% , 0.43% and average recoveries （n =6 ） were 98.5% , 98.0% , 98. 1% respectively. A specific, sensitive and simple HPLC method was established for the determination of mixed cyclodextrin and quality control.

关 键 词：高效液相色谱法 Α-环糊精 Β-环糊精 Γ-环糊精 含量测定 

分 类 号：TQ461[化学工程—制药化工]