食品卫生微生物学检验培养基质量分析  

Quality Analysis of Microbial Culture Media for Food Hygienic Detection

作　　者：张颖辉[1,2,3] 吴清平[2] 张菊梅[2] 刘秀梅[4] 蔡芷荷[5] 

机构地区：[1]中国科学院武汉病毒研究所,湖北武汉430071 [2]广东省微生物研究所广东省菌种保藏与应用重点实验室,广东广州510070 [3]中国科学院研究生院,北京100049 [4]中国疾病预防控制中心营养与食品安全所,北京100050 [5]广东环凯微生物科技有限公司,广东广州510663

出　　处：《微生物学通报》2009年第11期1723-1726,共4页Microbiology China

基　　金：广东省科技计划项目资助(No.2004B80502003;2005B80501007)

摘　　要：为探讨培养基ISO国际标准对国内培养基质控的等效性,选择国内外4家培养基生产厂家的霉菌培养基、酵母菌培养基和显色培养基,从质控菌株生长率、抑菌效果等方面进行质量分析比对试验。生长率质控标准菌株在各种霉菌培养基、酵母菌培养基上的生长率均值均大于0.5,在显色培养基上的生长率均值均大于0.7,符合ISO国际标准。经统计分析,试验的4家培养基厂家生产的培养基质量水平差异不显著(P>0.05),这为将新出台的培养基国家标准拟等效采用培养基ISO国际标准提供了基础材料和依据。To explore the equivalence of ISO culture media standards for domestic culture media quality control, fungus culture and chromogenic media were chosen from four domestic and foreign manufacturers, compared in quality control straifi productivity ratio and bacteriostatic effect. The productivity ratioes of productivity reference stains on fungus culture are all above 0.5, and that on chromogenic media are all above 0.7, which comply with ISO international standards. After statistical analysis of these results, there are no significant difference among these culture media （P 〉 0.05） chosen from the four culture media manufacturers in this study, which provide basic materials and basis for new national standard that is about to published to equivalent adopting ISO culture media standards.

关 键 词：培养基 生长率 抑菌效果 质量 食品卫生 

分 类 号：R155[医药卫生—营养与食品卫生学]