榆黄菇优良新菌株的选育  

Breeding of A Novel Pleurotus citrinopileatus Strain

作　　者：蔡德华[1] 张秋胜[1] 董洪新[1] 王香兰 刘淑芳 王艳杰[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]海阳市第一中学,山东海阳265100 [3]海阳市教师进修学校,山东海阳265100

出　　处：《食品科学》2009年第21期301-304,共4页Food Science

基　　金：山东省农业良种工程项目(鲁科农字[2005]99号);山东省教育厅科技计划项目(J06N02);鲁东大学人才基金项目(LY20063303);鲁东大学创新团队建设项目

摘　　要：为研究60Co-γ射线对榆黄菇菌丝遗传诱变的影响,采用不同的辐照剂量和不同的辐照剂量率对出发菌株榆黄菇818的尖端菌丝进行辐照处理。结果表明:在辐照剂量为600Gy、辐照剂量率为43.5Gy/h的条件下,菌丝再生后进行挑选,获得了生长优良的诱变菌株。将诱变菌株与出发菌株进行生长对比实验,诱变菌株菌丝生长速度平均提高21.1%;经拮抗实验、酯酶和过氧化物酶同工酶实验,证明诱变菌株遗传物质发生了变化,已不同于出发菌株,具有种的特异性;经连续转接6代进行固体培养,诱变菌株优良的生长性能得到了保持,具有遗传稳定性。The effect of 60Co-γ irradiation with different doses and dose rates on mycelial growth of Pleurotus citrinopileatus was investigated in this study. Mutant strains of Pleurotus citrinopileatus with high growth rate were obtained through 60Co-γ irradiation at 600 Gy and 43.5 Gy/h dose rate. After the regeneration of mycelia, a novel strain having the highest growth rate was screened, which showed an average increase of 21.1% when compared with that of the parental strain. Results of antagonistic test, esterase isozyme and peroxidase isozyme eleetrophoresis revealed significant genetic differences between the mutant strain and its parent stain. After six generations of continuous cultivation, the mutant strain kept the good growth vigor, thereby displaying high genetic stability.

关 键 词：榆黄菇 诱变 选育 

分 类 号：S646[农业科学—蔬菜学]