枯草芽孢杆菌固态发酵麻疯树废弃饼粕产蛋白酶初步研究  

Study on Protease Production from De-oiled Jatropha curcas Seed Cakes by Bacillus subtilis in Solid-state Fermentation

作　　者：杜亚菲[1,2] 吴远根[1,2] 吴洁[2] 满敬杰[2] 郭云兰[2] 邱树毅[1,2] 

机构地区：[1]贵州大学贵州省发酵工程与生物制药重点实验室,贵州贵阳550003 [2]贵州大学化学与化工学院,贵州贵阳550003

出　　处：《贵州农业科学》2009年第11期83-85,共3页Guizhou Agricultural Sciences

基　　金：教育部"春晖"计划"微生物发酵辅助提取麻疯树油脂并耦联产饲料研究"(Z2007-1-52017);贵州省科学技术基金"麻疯树果提油后饼粕的发酵脱毒与综合利用研究"[黔科合J字(2007)2035];国家大学生创新性实验计划"固态发酵麻疯树饼粕产蛋白酶及酶学性质的研究"[贵大国创字(2008)017];贵州大学大学生创新性实验计划"固态发酵麻疯树饼粕产蛋白酶的初步研究"[贵大创字(2008)114]

摘　　要：为寻找出麻疯树制备生物柴油后废弃饼粕合理利用的有效途径,利用枯草芽孢杆菌作为发酵菌株,以提油后的麻疯树饼粕作为培养基,采用固态发酵方式进行产蛋白酶试验。结果表明:控制培养基相对湿度为150%,37℃下发酵2 d,蛋白酶产量达到3 284 U/g;向培养基中添加15%葡萄糖,则蛋白酶产量达到12 108 U/g,比对照组提高3.6倍;向培养基中添加氮源后,蛋白酶产量反而下降。The test of protease production from de-oiled Jatropha curcas seed cake （JCSC） by Bacillus subtilis in solid-state fermentation was conducted to find the effective ways to rationally utilize de-oiled seed cakes of Jatropha curcas. The results showed that the protease yield was 3284U/g when 150% relative humidity of JCSC was fermented by Bacillus subtilis at 37℃ for 2 days. The protease yield could be up to 12108U/g when 15% glucose was added in JCSC and was 3. 6 times higher than CK. The protease yield decreased when nitrogen source was added in JCSC.

关 键 词：麻疯树饼粕 枯草芽孢杆菌 蛋白酶 固态发酵 

分 类 号：Q939.97[生物学—微生物学]