表面活性剂偶联原位萃取发酵对阿维拉霉素产量的影响  

Effects of surfactants coupled to in-situ extraction fermentation on the yield of avilamycin

作　　者：李政[1] 闫达中[1] 陶兴无[1] 张宇廷[1] 刘晓龙[1] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023

出　　处：《中国酿造》2009年第12期70-72,共3页China Brewing

摘　　要：以本实验室筛选获得的绿色产色链霉菌(Streptomyces viridochromogenes)SVT-12为发酵工艺探讨的出发菌株,根据阿维拉霉素为细胞内产物的原理,通过向培养基中添加大孔树脂X-5和表面活性剂吐温-80,尝试通过大孔树脂吸附发酵液中的阿维拉霉素,利用表面活性剂改变细胞膜的通透性,降低阿维拉霉素代谢合成的负反馈抑制作用,提高抗生素发酵产量。结果表明,培养基中添加大孔树脂X-5和0.25%的吐温-80时可显著提高抗生素的发酵产量。In this study, Streptomyces viridochromogenes SVT-12 was used as a pure starter to carry on fermentation process. Considering that avilamycin is a kind of intracellular products, macroporous resin was added to adsorb avilamycin selevtively, and tween-80 was added into the medium to change bacterial membrane permeability and decreased the negative feedback inhibition. The results showed that through addition of macroporous resin X-5 and tween-80 （0.25%）, the yield of avilamycin was significantly increased.

关 键 词：阿维拉霉素 发酵 表面活性剂 大孔树脂 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]