胰蛋白酶限制性水解脱酚棉籽蛋白的研究  

Partially hydrolysis of de-gossypol cottonseed protein with trypsinase

作　　者：张汆[1] 许雪峰[1] 李敏[1] 孙艳辉[1] 贾小丽[1] 

机构地区：[1]滁州学院化学与生命科学系,安徽滁州239012

出　　处：《中国油脂》2009年第12期26-29,共4页China Oils and Fats

基　　金：安徽高校省级自然科学研究项目(KJ2009B146);安徽省应用化学省级重点学科建设项目(200802187C)

摘　　要：采用胰蛋白酶对脱酚棉籽蛋白进行限制性酶解，以改善棉籽蛋白在中性条件下的溶解性。以酶解棉籽蛋白水解度和溶解度为指标，对胰蛋白酶水解脱酚棉籽蛋白的条件进行了优化。结果表明，胰蛋白酶水解脱酚棉籽蛋白的优化条件为：酶底比2：1（50000U／g），底物质量浓度100g／L，pH10，于20℃下酶解3h。在此条件下，酶解棉籽蛋白的水解度为10．87％，溶解度为63．48％。与酶解前相比，溶解度提高了43．82％。溶解度分析结果表明，限制性酶解棉籽蛋白在pH1～11范围内均具有较好的溶解度，仍然保持了棉籽蛋白酸溶性的特点。The partially hydrolysis of de-gossypol cottonseed protein by trypsinase was investigated to improve the solubility of cottonseed protein in neutral pH solution. The hydrolysis parameters were analyzed and optimized by determining the degree of hydrolysis and solubility. The results indicated that the optimal conditions were as follows ： ratio of enzyme to substrate 2 ： 1 （ 50 000 U/g）, substrate mass concentration 100 g/L, pH 10, temperature 20 ℃, time 3 h. Under the conditions, the degree of hydrolysis and solubility of zymolytic cottonseed protein were 10.87% and 63.48% ,respectively. The solubility of cottonseed pro- tein increased by 43.82% after enzymolysis. The zymolytic cottonseed protein had higher solubility in pH 1 -11, and it still maintained the acid-soluble character.

关 键 词：胰蛋白酶 限制性酶解 棉籽蛋白 溶解度 

分 类 号：TS201.21[轻工技术与工程—食品科学]