无溶剂体系脂肪酶催化大豆油水解反应动力学——界面面积与反应初速度  

Kinetic of lipase-catalyzed hydrolysis of soy oil in a solvent free system:study on the interfacial area and initial rates

作　　者：黄健花[1] 刘亚轩[1] 宋志华[1] 金青哲[1] 刘元法[1] 王兴国[1] 

机构地区：[1]江南大学食品学院,食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《中国油脂》2009年第12期34-38,共5页China Oils and Fats

基　　金：国家"十一五"支撑计划项目(2006OBAD27B03);教育部博士点基金项目(20070295004)资助

摘　　要：对无溶剂体系中C.lipolytica脂肪酶催化大豆油水解反应过程中的油水界面面积与反应初速度进行了研究。对超声波反应器和振荡水浴两种不同的方式进行了比较,超声波较振荡水浴具有更好的分散作用,油滴粒径更小,界面面积更大;提高振荡速度和超声波强度,均可以增大界面面积;振荡水浴中,界面面积随油相体积分数的增加而减少;超声波反应器中,界面面积随油相体积分数的增加先增加后减少;反应初速度与界面面积呈线性相关,反应初速度随界面面积的增加而增大,当油相体积分数为0.7,超声波强度为1.64 W/cm2时,反应初速度最大,为161 mol/(m3.m in)。The interfacial area between the oil and the aqueous solution and the initial rate in the lipase - catalyzed hydrolysis of soy oil in a solvent free system were studied, and the hydrolysis ways in shaking bath and in ultrasonic bath were compared. Compared with shaking bath, ultrasonic bath was found to be a more effective procedure to disperse the oil in water. Larger interfacial area and smaller drop size could be got in ultrasonic bath. The higher interfacial area between the oil phase and the aqueous phase could be got with higher ultrasonic power and higher shaking speed. In shaking bath, the interfacial area decreased with the increase of oil volume fraction. With the increase of oil volume fraction, the interfacial area increased firstly and then decreased in ultrasonic bath. The initial rate of hydrolysis increased with the increase of interfacial area, and a linear correlation was found between the initial rate and the interfacial area. The highest initial rate was 161 mol/（ m3 · min） under the conditions of the oil volume fraction of 0. 7 in ultrasonic bath with the power of 1.64 W/cm2.

关 键 词：脂肪酶 大豆油 水解 界面面积 初速度 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]