化学发光法检测保健食品中SOD活性的研究  

The research on superoxide dismutase activity in health foods with chemical luminescence method

作　　者：罗速 张吉林 兰英 

机构地区：[1]吉林医学院生化教研室,吉林 [2]吉林医学院生化检验教研室

出　　处：《吉林医学院学报》1998年第1期3-5,共3页

基　　金：卫生部95制标项目

摘　　要：目的：建立保健食品ＳＯＤ活性检测的标准。方法：采用化学发光法测定保健食品（花粉、茶等）中ＳＯＤ活性，及其试验条件的研究。即黄嘌呤氧化酶、次黄嘌呤、鲁咪诺、温度等因素对ＳＯＤ活性测定体系的影响，进行该方法的精密度试验，其ＣＶ（％）为２．３。结果：方法重现性好，灵敏高度，操作简便，检测迅速。结论：是监测ＳＯＤ型保健食品ＳＯＤ活性的可靠方法。Objective:To establish the detected standard of SOD activity in health foods. Methods:The Superoxide dismutase (SOD) activity in the health foods (pollen, tea),vas detected with the chemical lurninescence, and the experimental conditions were studied, too. The factors, ie, xanthine xanthine oxidase hypoxanthine, luminol and the temperature had individually influences upon the determined system of SOD activity We also made the precision test. The C V of the method was 2.3%. Results:The reappearance was very good, the semsitivity was high and the determination was rapid. Conclusion: The chemical Luminescence was a reliable method to monitor SOD activity in the health foods.

关 键 词：SOD 黄嘌呤氧化酶 次黄嘌呤 鲁咪诺 保健食品 

分 类 号：R151.3[医药卫生—营养与食品卫生学]