哈尔滨市鲜肉中单核细胞增生性李斯特菌的分离鉴定及耐药性分析  

Isolation and identification of antibiotic resistance of Listeria monocytogenes isolated from meat in Harbin

作　　者：尹录[1,2] 杜艳芬[2] 赫明雷[1,2] 司微[2] 刘慧芳[2] 王春来[2] 彭玮[1,2] 王聃[2,3] 李继昌[1] 邵美丽[1] 刘思国[2] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/动物细菌病研究室,黑龙江哈尔滨150001 [3]延边大学,吉林龙井133400

出　　处：《中国预防兽医学报》2009年第12期929-932,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省科技厅青年基金(QC08C100);东北农业大学博士启动基金;农业部公益行业科研专项(200803054);黑龙江省博士后基金(LBH-Z07015)

摘　　要：为了解哈尔滨市单核细胞增生性李斯特菌(Lm)的污染状况及耐药状况。在哈尔滨市市场随机采集158份鲜肉样品,采用显色培养基分离,API试剂条和PCR鉴定等方法对样品中的Lm进行分离鉴定,并通过Kirby-Barer法测定分离菌株对24种抗生素的耐药性。结果从鲜肉中共分离到Lm23株,检出率为14.56%,其中鲜猪肉检出率最高,达20.00%(14/70);23株分离菌株中耐药菌株为22株,耐药率高达95.65%。这表明哈尔滨市鲜肉中存在一定程度的Lm污染,并且分离菌株存在较严重的耐药现象。应加强控制动物饲料亚治疗抗生素的使用并严格遵守休药期,防止耐药菌株产生进而控制食源性疾病的发生。To investigate the contamination and drug resistance of Listeria monocytogenes in meat, 158 flesh meat samples were randomly collected flom meat markets in Harbin, and 23 Listeria monocytogenes strains （14.6 % positive） were isolated and characterized by Coloration medium, API reagent strip and PCR. The contamination in raw pork is the highest, with 20.00 % （14/70） detected positive. The drug resistance of isolated strains was tested against antibacterial drugs by Kirby-Barer method, and all but one （95.65 %） displayed drug resistance. The results showed the Listeria monocytogencs contamination in flesh meat was evident in Harbin and drug resistant rate was very serious. In order to reduce the drug resistance pathogens it is necessary to control and reduce the use of antibiotics in animal feedstuffs.

关 键 词：哈尔滨 鲜肉 单核细胞增生性李斯特菌 分离鉴定 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]