荷叶生物碱提取工艺响应曲面优化研究  

Optimization of Alkaloid Extraction from Lotus Leaves by Response Surface Method

作　　者：肖娟[1] 孙智达[1] 谢笔钧[1] 杨尔宁[1] 

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品科学》2009年第22期157-161,共5页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAD27B09-4)

摘　　要：以荷叶为材料,在提取温度、提取时间、乙醇体积分数和液料比4个单因素试验的基础上,利用响应曲面试验设计法对荷叶生物碱提取条件进行研究,并通过回归方程和响应曲面,得到的最佳提取工艺为:提取温度84℃,提取时间2.01h,乙醇体积分数90.65%,液料比29.74:1(ml/g)。验证实验结果显示,此条件下荷叶生物碱提取率为0.858%。荷叶碱在6.40～32.00μg/ml范围内具有良好的线性关系,r=0.992,荷叶中荷叶碱占荷叶生物碱提取物的比例为7.952%,荷叶中荷叶碱的含量为0.186%。Optimal condition for alkaloid extraction from lotus leaves was investigated through response surface method based on single factor experiments to study the effect of extraction temper ature,extraction time,alcohol concentration and liquid-material ratio on extraction rate.The optimal extraction condition was 84 ℃ of extraction temperature,2.01 h of extraction time,90.65% of alcohol,and 29.74：1（ml/g） of liquid-material ratio.Results indicated that alkaloid extraction from lotus leaves was 0.858% under this optimal condition.A linear was observed in the range of 6.40-32.00 μ g/ml（r = 0.992）.The content of nuciferine in alkaloids from lotus leaves was 7.952%.

关 键 词：荷叶 生物碱 提取工艺 荷叶碱 响应曲面法 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]