转苷酶活性测定方法  

Determination of Glucosyltransferase Activity

作　　者：陈烨璞[1] 汪云[1] 胡学铮[1] 

机构地区：[1]无锡轻工大学化学工程系

出　　处：《无锡轻工大学学报（食品与生物技术）》1998年第3期86-89,共4页Journal of Wuxi University of Light Industry

摘　　要：对测定糖化酶中葡萄糖苷转移酶（转苷酶）酶活的方法进行了研究。采用α－ＭＧ为底物，用ｋｎｉｇｈｔ－Ａｌｅｎ法测定转苷酶催化α－ＭＧ分解产生的葡萄糖，计算得转苷酶酶活值。通过条件试验，找到了底物浓度、碱性铜试剂用量、加热时间、滴定剂浓度等的最佳范围。并测定了３×１０４，５×１０４，６×１０４μｍｏｌ／ｍｉｎ糖化酶粉剂和发酵液中的转苷酶活性，做了酶法、光度法与该方法的对比试验。In this paper, a determination method of glucosyltransferase activity in saccharogenic enzyme is developed. Taking α MG as a substrate, glucose decomposed from α MG catalyzed by glucosyltransferas is determined with Knight Allen method and the activities of glucosyltransferase is calculated. By conditional test, the best limits are obtained for concerntration of the substrate, heating period, and concentration of titrant. The activities of glucosyltransferase in saccharogenic enzyme powder and fermentation liquid per 3000, 5000, 6000 unit are determined. The result suggests that this determination method has the advantages of accuracy, quickness and simplicity.

关 键 词：葡萄糖苷转移酶 糖化酶 酶活性 转苷酶 

分 类 号：TQ925.4[轻工技术与工程—发酵工程] TS245.4