番茄促分裂素原活化蛋白激酶的研究进展与生物信息学分析  

Research Progress and Bioinformatics Analysis of Tomato MAPKs

作　　者：赵瑞瑞[1] 丁洋[1] 申琳[1,2] 赵珩[1] 生吉萍[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]新疆农业大学食品科学学院,新疆乌鲁木齐830052

出　　处：《食品科学》2009年第23期479-483,共5页Food Science

基　　金：国家自然科学基金项目(30671471;30571291);公益性行业科技项目(200803033)

摘　　要：促分裂素原活化蛋白激酶(mitogen-activated protein kinases,MAPK、MPK或MK)级联是一种广泛存在于真核生物中的信号传导途径。同动物和酵母类似,植物细胞的MAPK级联也是由MAPKKK(MEKK,MKKK),MAPKK(MEK,MKK),MAPK三个级别的激酶对其下游蛋白的磷酸化构成的,最终对细胞生长、发育及抗性等过程中的效应分子进行调控。本文介绍了番茄目前的测序情况,以及番茄(Lycopersicon esculentum)MAPK(LeMAPK,LeMPK)的研究进展,根据MAPK序列在进化过程中的保守性,用模式植物拟南芥(Arabidopsis thaliana)MAPK(AtMAPK,AtMPK)基因序列在番茄的转录序列中搜索,得到18个LeMAPK条目,其中两条是新的LeMAPK,并与AtMAPK进行比对和归类。The plant mitogen-activated protein kinases （MAPK） pathwaqys transduce a large variety of external signals, leading to a wide range of cellular responses, including growth, differentiation, stress reactions. In this review, we emphasized recent findings on the MAPK involvement related to stress tolerance in tomato （Lycopersicon esculentum MAPK, LeMAPK）. According to the conservation of the MAPK sequences in different plants, we blasted the candidate LeMAPKs with the Arabidopsis （Arabidopsis thaliana） MAPK （AtMAPK, AtMPK） sequences and got 18 LeMAPKs with two new sequences. The sequences of LeMAPKs and AtMAPKs were alignmented. In addition, the potential LeMAPKs were primarily documented by informatic analysis, which were divided into five groups based on the phylogenetic analysis.

关 键 词：植物 MAPK 磷酸化 激活 环境胁迫 番茄 

分 类 号：S641.2[农业科学—蔬菜学]