改良DEAE-纤维素法制备龙须菜琼胶糖研究  

Preparation of Gracilaria lemaneiformis Agarose by Modified DEAE-cellulose Method

作　　者：戚勃[1] 杨贤庆[1] 赵永强[1,2] 李来好[1] 陈胜军[1] 岑剑伟[1] 马海霞[1] 刁石强[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]广东海洋大学食品科技学院,广东湛江524025

出　　处：《食品科学》2009年第24期118-121,共4页Food Science

基　　金：国家高技术研究发展计划项目(2007AA10Z345);农业部公益性行业(农业)专项(200903030-E);广东省科技计划项目(2007A032600003);广东省海洋渔业科技推广专项(A200899E01);中国水产科学研究院南海水产研究所中央级公益性科研院所专项资金项目(2007ZD06)

摘　　要：以龙须菜琼胶为原料,分别采用KMnO4-H2C2O4法漂白、乙醇处理和DEAE-纤维素纯化等工艺制备生化级琼胶糖,并采用正交试验设计对工艺参数进行优化,对琼胶糖的理化指标和电泳性能进行测定。结果表明:漂白实验的最佳工艺条件为KMnO4浓度0.10%、pH6.0、漂白时间5min、H2C2O4浓度0.30%,漂白后琼胶白度(HW值)为82.98、凝胶强度为1083g/cm2;乙醇处理的最佳工艺条件为料液比1:40(g/ml)、乙醇体积分数60%、处理时间6h,处理后琼胶透明度(透光率)为50.2%。制备的琼胶糖灰分含量为0.25%、凝胶强度为1127g/cm2、硫酸基含量为0.24%;结晶紫电泳、电内渗测定和基因组DNA电泳实验表明,改良DEAE-纤维素法制备的琼胶糖具有优异的电泳性能,适用于生物化学和分子生物学的凝胶电泳研究。Gracilaria lemaneiformis agarose was prepared through KMnO4-H2C2O4 bleaching, ethanol soaking and DEAE cellulose purification, respectively. Orthogonal experiments were used to optimize processing parameters. Physical and chemical index, and electrophoresis performance of agarose were also determined. Results showed that the optimal condition for bleaching was 0.10% KMnO4, 0.30% H2C2O4, pH 6.0, bleaching time for 5 min. After bleaching treatment, whiteness （HW value） of agar was 82.98 and gel strength was 1083 g/cm^2. The optimal condition for ethanol soaking was 1：40 of solid-liquid ratio, 60% ethanol, treatment time for 6 h. After ethanol soaking, transparency of 1.0% agarose （transmittance value） was 50.2%. The ash content, gel strength and sulfate group content in agarose prepared by the optimal method were 0.25%, 1127 g/cm^2 and 0.24%, respectively. Gentian violet electrophoresis, electroendosmosis and genome DNA gel electrophoresis were also performed to reveal that the agarose prepared by modified DEAE-cellulose method was suitable for biochemistry and molecular biology electrophoresis.

关 键 词：琼胶糖 DEAE-纤维素 DNA凝胶电泳 

分 类 号：Q539.9[生物学—生物化学] TS245.9[轻工技术与工程—制糖工程]