ASE-GPC-GC法测定大豆及豆制品中六六六、滴滴涕农药残留  

Simultaneous Determination of 8 HCH and DDT Residues in Soybean and Soybean Products with ASE-GPC Coupled with Gas Chromatography

作　　者：魏冬旭[1,2] 江连洲[1,3] 郭伟[1] 孟冰冰 胡少新[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]黑龙江出入境检验检疫局检验检疫技术中心,黑龙江哈尔滨150001 [3]中国大豆工程技术中心,黑龙江哈尔滨150086

出　　处：《食品科学》2009年第24期351-354,共4页Food Science

摘　　要：建立一种同时测定大豆及豆制品中α-HCH、β-HCH、γ-HCH、δ-HCH、ρ,ρ′-DDE、ρ,ρ′-DDD、ο,ρ′-DDT、ρ,ρ′-DDT8种有机氯农药残留的气相色谱分析方法。样品经加速溶剂系统萃取(ASE)和凝胶渗透色谱净化(GPC)后,采用气相色谱配备电子捕获检测器进行分离测定。0.005～0.100mg/kg范围内各残留药物线性良好,相关系数r均在0.9990以上;在0.005、0.020、0.050mg/kg3个加标水平下进行了方法验证,平均回收率为78.3%～107.6%,相对标准偏差为2.1%～5.5%;方法检测限(LOD)为0.2～1.8μg/kg。该方法重现性好、灵敏度高、分析迅速,各项指标均满足国内外法规要求,适用于大豆及豆制品中六六六和滴滴涕残留的分析检测。A method for the determination of 8 HCH and DDT in soybean and soybean products was established with accelerated solvent extraction （ASE）, auto gel permeation chromatography purification （GPC） and gas chromatography quantification. The calibration curves presented good linear relationship in the range of 0.005-0.100 mg/kg, and the correlation coefficients were above 0.9990 for all compounds. Recoveries of all added were 78.3%-107.6% respectively, and relative standard deviations （RSD） were 2.1%-5.5% with spiked at 0.005, 0.020, 0.050 mg/kg of HCH and DDT in blank samples. The limit of detection of the method were 0.2-1.8μg/kg. The method was of good reproducibility, high sensitivity, shorter analysis time, and its performance could meet the requirements of the domestic and international legislation.

关 键 词：大豆及豆制品 加速溶剂萃取 凝胶渗透色谱 气相色谱 六六六 滴滴涕 

分 类 号：TS207.53[轻工技术与工程—食品科学]