乳制品中牛IgG含量间接竞争性ELISA检测方法的研究  

Development of a competitive indirect enzyme linked immunoassay for the analysis of bovine immunoglobulin G in milk products

作　　者：高东微[1] 马孝斌 孙远明[3] 陈源树[1] 

机构地区：[1]广东检验检疫技术中心,广东广州510623 [2]北京望尔生物技术有限公司,北京102206 [3]华南农业大学食品学院,广东广州510630

出　　处：《食品工业科技》2010年第1期395-398,共4页Science and Technology of Food Industry

基　　金：国家质检总局科技计划项目(2009IK269)支持

摘　　要：建立了牛初乳制品和添加了牛初乳成分的乳制品中牛IgG含量间接竞争性酶联免疫吸附测定方法,主要研究步骤包括:制备牛IgG保守区(Fc段),以Fc段为免疫原免疫Balb/C小鼠,取其脾细胞进行细胞融合,制备特异性单克隆抗体及合成适合实际测定用途的ELISA试剂盒。应用本研究建立的方法对标准品和实际样品进行牛IgG含量检测的结果表明,该方法回收率在78.9%～117.5%,批内变异系数小于10%,批间变异系数小于15%,检测结果稳定可靠,可满足目前国内牛初乳制品和添加了牛初乳成分的乳制品品质监控的需要。A competitive indirect ELISA for the analysis of bovine immunoglobulin G in bovine colostrum and other colostrum contained milk products was established.The main steps of the research included the preparation of Fc segment from bovine immunoglobulin G, the immunization of Balb/C mice with Fc segment, the fusion of the spleen cells from Balb/C mice, the screening of specific monoclonal antibodies and the assembly of ELISA reagents suitable for practical analysis.The results of bovine IgG analysis on standards and samples using the method established indicated that the recovery rate was 78.9%-117.5%, while the intra-assay C.V.was less than 10% and inter-assay C.V.was less than 15% ,which demonstrated the stability and reliability, and showed a high applicability of the method in the quality control of domestic bovine colostrum and other colostrum contained milk products.

关 键 词：牛免疫球蛋白G 牛初乳 酶联免疫吸附测定 

分 类 号：TS207.3[轻工技术与工程—食品科学]