高产纤维素酶生产菌的筛选及诱变育种  

Screening and Mutagenesis of High Cellulase Activity Strain

作　　者：方尚玲[1] 杨丹丹[1] 钱志伟[1] 李小强[1] 陈茂彬[1] 

机构地区：[1]发酵工程省部共建教育部重点实验室,湖北工业大学生物工程学院,湖北武汉430068

出　　处：《食品与发酵科技》2010年第1期13-17,共5页Food and Fermentation Science & Technology

基　　金：湖北省自然科学基金项目(2008CDB063);湖北工业大学博士启动基金(BSQD0910)资助

摘　　要：本研究分离筛选出1株产纤维素酶的木霉(CMC酶活609.51U/g干曲、FPA酶活133.85U/g干曲),以该木霉为出发菌,经紫外线及原生质体紫外线复合诱变育种,获得一株纤维素酶活力较高生产性能稳定的菌株,其CMC酶活达到1332.90U/g干曲,FPA酶活达到301.61U/g干曲,分别是原始菌株的2.19倍,2.25倍。Use the Tr/choderma （CMC enzyme activity was 609.51U/g dry medium,FPA enzyme activity was 133.85U/g dry medium） as the original strain, a strain with steady high activity cellulase was obtained after UV mutation and protoplast mutagenesis. The CMC enzyme activity was 1332.90U/g dry medium,the FPA enzyme activity was 301.61U/g dry medium which were 2.19 times and 2.25 times higher than the raw strain respectively.

关 键 词：纤维素酶 原生质体 木霉 

分 类 号：TS201.3[轻工技术与工程—食品科学] TS261.1[轻工技术与工程—食品科学与工程]