不同方法萃取蜂胶挥发油组成及抑菌作用的研究  

Propolis Essential Oil Extracted by Different Methods: Chemical Composition Analysis and Antibacterial Activity Evaluation

作　　者：徐响[1,2] 董捷[1,2] 丁小宇[1] 杨佳林[1] 孙丽萍[1,2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]农业部国家蜂产品加工专业分中心,北京100093

出　　处：《食品科学》2010年第3期60-63,共4页Food Science

基　　金：农业部现代农业(蜂)产业技术体系建设专项资金项目(NYCYTX-43);中国农业科学院院本级基本科研业务费项目(0032007222)

摘　　要：采用水蒸气蒸馏法和超临界二氧化碳萃取法获得了蜂胶挥发油。GC-MS分析并鉴定出50种化合物,两种提取方法所得蜂胶挥发油在成分组成上存在显著差异。抑菌实验表明,两种提取方法获得的挥发油对金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌具有很强的抑制作用,而超临界法萃取的挥发油的抑菌效果强于水蒸气蒸馏法。In this work,propolis essential oil was obtained by steam distillation extraction and supercritical CO2 extraction,respectively.Added together,50 components in resulting propolis essential oils were identified by GC-MS method.Propolis essential oils extracted by both methods exhibited strong inhibition effects against Staphylococcus aureus,Bacillus subtilis and Pseudomonas aeruginosa in vitro.Stronger inhibition effect of propolis essential oil extracted by supercritical CO2 extraction on the three species of bacteria than that of propolis essential oil extracted by steam distillation extraction was observed.

关 键 词：超临界二氧化碳萃取法 水蒸气蒸馏法 蜂胶 抑菌作用 

分 类 号：S896[农业科学—特种经济动物饲养]