板栗壳中多酚的提取及体外抗氧化性研究  

Study on Extraction and Antioxidation Property of Polyphenol from Chestnut Shells

作　　者：李金凤[1] 段玉清[1] 马海乐[1] 张海晖[1] 徐菲菲[1] 黄六容[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《林产化学与工业》2010年第1期53-58,共6页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(30270938);江苏省教育厅基金项目(05KJB550011);江苏大学人才基金项目(05JDG013)

摘　　要：研究了不同因素对板栗壳多酚提取效果的影响，并对不同条件下板栗壳多酚的抗氧化性进行了研究。结果表明，板栗壳多酚的最佳提取条件为：乙醇体积分数59．7％，料液比1：18（g：mL），浸提温度52．6℃，浸提次数3次。在此最优工艺条件下板栗壳多酚提取得率预测值为76．0mg／g，实验值75．9mg／g。板栗壳多酚具有较强的清除自由基能力，在25-200mg／L范围内，随质量浓度升高其还原能力和清除二苯基苦基肼自由基（DPPH·）、羟基自由基（·OH）、超氧阴离子自由基（O2^-·）的能力逐渐增强，当200mg／L时其还原能力达0．841，DPPH·抑制率达89．7％，均高于同质量浓度的2，6-二叔丁基对甲酚（BHT），但低于同质量浓度Vc，对O2^-和·OH的清除率分别为93．2％和94．0％，均高于同质量浓度BHT和Vc。The effects of different factors on extraction of polyphenol from chestnut shell were studied, and the antioxidation pro- perty of polyphenol of chestnut shell was also studied. The results showed that the optimum extraction conditions were ： ethanol volume fraction 59.7 % , solid-liquid ratio 1 ： 18 （g： mL）, extraction temperature 52.6 ~C and extract 3 times. The predicted yield of chestnut shell polyphenol is 76.0 mg/g and the test result is 75.9 mg/g. Polyphenol in chestnut shell has strong ability to eliminate free radicals. The abilities of reducing and removing DPPH. , ＂OH, Of＂ gradually increased with the increase of concentration in the range of 25 to 200 mg/L. The ability of reduction reached 0.841, and the rate of inhibition reached 89.7 % at 200 mg/L, which are higher than those of 2,6-di-tert-butyl-p-cresol （BHT） but lower than those of Vc, at similar mass concentration. The removal rates of Of. and .OH are 93.2 % and 94.0 % , respectively, at concentration of 200 mg/L, which are higher than BHT and Vc.

关 键 词：板栗壳 多酚 提取 抗氧化性 

分 类 号：TQ351[化学工程]