一株耐热脂肪酶产生菌的筛选及酶学性质研究  

The Screening of Thermostable Lipase-producing Bacterium and Study on Properties of Lipase

作　　者：赵春雷[1] 闫丽娟[1] 谢振荣[1] 高润池[1] 李俊[1,2,3] 唐湘华[1,2,3] 黄遵锡[1,2,3] 

机构地区：[1]云南师范大学生命科学学院,昆明650092 [2]云南师范大学酶工程重点实验室,昆明650092 [3]生物能源持续开发利用教育部工程研究中心,昆明650092

出　　处：《生物技术通报》2010年第2期184-188,共5页Biotechnology Bulletin

基　　金：国家"863"计划项目(2008AA02Z202)

摘　　要：从云南省富油地采取了60份土样中,利用透明圈法筛选出一株耐热脂肪酶产生菌。对其酶学性质和发酵条件进行了研究,酶学性质表明,该酶最适作用温度为50℃,最适pH6.0,在pH3.0-8.0范围内稳定,在60℃保温60 min酶活还保留70%;70℃保温60 min残余50%;具有良好的热稳定性;不同金属离子有不同的作用,Ca+,K+对酶有激活作用,Fe3+、Pb2+、Mn2、Cu2+、Al3+、Zn2+对酶活有抑制作用。EDTA对酶影响不大。产酶最佳条件为:MgSO4.7H2O 0.05 g,K2HPO40.1 g,CaCO30.25 g,可溶性淀粉2.5 g,大豆粉2.5 g,装液量50 mL。这株细菌通过培养基优化酶活达到20.3 U/mL。From 60 oil-rich soil samples in Yunnan province,a thermostable lipase-producing bacterium was screened by using a transparent circle method. The nature of its enzymatic and fermentation conditions was studied. Enzymatic properties showed that the enzyme optimal temperature is 50℃ ,optimum pH6.0, the lipase activity is stable at pH3.0 - 8.0, and enzymatic activity retained 70% at 60℃ for 60 minutes. The residual is 50% at 70℃ for 60 minutes. Different metal ions showed diverse effects on the lipase activities, Ca^＋ , K^＋ increased lipase activities,whereas Fe^3＋ , Pb^2＋ , Mn^2＋ , Cu^2＋ , Al^3＋ , Zn^2＋ can inhibit the enzyme activity. EDTA effect on the enzyme little bit. Optimum conditions for enzyme production ： MgSO47H2O 0.05 g, K2HPO4 0. 1 g, CaCO30.25 g, soluble starch 2.5 g, soybean powder 2.5 g,liquid volume 50 mL. This strain of bacteria of enzymatic activity can achieve 20.3 U/ml through the medium optimization.

关 键 词：脂肪酶产生菌 产酶条件 酶学性质 

分 类 号：TQ925[轻工技术与工程—发酵工程]