DNA分子标记技术在酿酒酵母菌株遗传多样性分析中的应用  

Application of DNA Molecular Markers in Genetic Diversities of Saccharomyces cerevisiae Strains

作　　者：裴颖芳[1] 刘延琳[1] 

机构地区：[1]西北农林科技大学葡萄酒学院/陕西省葡萄-葡萄酒工程中心,陕西杨凌712100

出　　处：《中国农业科学》2010年第5期1023-1030,共8页Scientia Agricultura Sinica

基　　金：现代农业产业技术体系建设专项资金资助项目(nycytx-30);国家"863"计划专项(2007AA10Z314)

摘　　要：本文介绍了RAPD、mtDNA-RFLP、微卫星DNA(microsatellite)、Interdelta指纹图谱以及线粒体DNACOX1基因指纹图谱等5种DNA分子标记技术的方法和原理及其优缺点,同时探讨了DNA分子标记技术在国内外酿酒酵母菌株区分中的应用状况与前景。The basic principles of the methods concerning five DNA molecular markers including random amplified polymorphic DNA （RAPD）, fragment length polyrnorphism of mitochondrial DNA （rntDNA-RFLP）, microsatellite DNA, interdelta fingerprinting and mtDNA COX1 gene fingerprinting were introduced in this paper, including illustration of both the advantages and the disadvantages of each method. Besides, the application and prospect of these DNA molecular markers in differentiation of Saccharomyces cerevisiae strains were discussed and reviewed.

关 键 词：酿酒酵母菌株 RAPD mtDNA—RFLP interdelta指纹图谱 COX1基因 微卫星DNA 

分 类 号：TS261.1[轻工技术与工程—发酵工程]