鸡内金粘多糖碱提工艺条件的探索  

Optimum Condition for Alkaline Extraction of Glycosaminoglycan from Endothelium corneum

作　　者：杜林繁[1] 卢家炯[1] 梅军 韦宇[1] 马森[1] 

机构地区：[1]广西大学轻工与食品工程学院,南宁530004 [2]郑州统一企业有限公司品保课,郑州450002

出　　处：《天然产物研究与开发》2009年第B05期144-149,共6页Natural Product Research and Development

摘　　要：以常用动物中药鸡内金为原料，用氢氧化钠为溶剂提取得到动物多糖（粘多糖）。碱提取法可以从组织中对粘多糖进行较完全的提取，但粘多糖分子有可能从断裂的一端被碱进一步降解，因此所用碱的浓度、碱解温度及时间都应优选。本实验研究了料液比，提取温度，提取时间和碱解浓度对提取率的影响。用响应曲面进行优化，最佳提取条件为：碱浓度1．5mol／L、提取温度45℃，提取时间50min，料液比1：25，此条件下提取率可达1．98％。The mucopolysaccharides were abstracted by alkali from Endothelium corneum. The effects of the materials fluids ration, extraction temperature ,time, alkali additive on extraction rate have been described. The optimizing extraction conditions were obtained from the response surface methodology ： the fluids materials ration was 1 ： 25, extraction temperature was 45 ℃, extraction time was 50 min, and alkali additive was 1.5 mol/L. The optimizing extraction rate was 1.98%.

关 键 词：鸡内金 粘多糖 碱提取 响应曲面 

分 类 号：R284.2[医药卫生—中药学] Q58[医药卫生—中医学]