南极海冰细菌中产低温 α-淀粉酶菌株的发酵和酶学特性的初步研究  

Studies on enzyme-producing conditions and enzymology property of cold-active α-amylase-producing strain from Antarctica

作　　者：史翠娟[1] 阚光锋[1] 雷振环[1] 钱文佳[1] 刘胜浩[2] 

机构地区：[1]哈尔滨工业大学(威海)海洋学院,山东威海264209 [2]国家海洋局海洋生物活性物质重点实验室,山东青岛266061

出　　处：《工业微生物》2010年第1期55-59,共5页Industrial Microbiology

基　　金：山东省自然科学基金(Y2007D57);威海市科技计划(2006B122);国家海洋局海洋生物活性物质重点实验室开放课题(IMOQ20080002)

摘　　要：从147株南极海冰细菌中筛选出一株高产低温a-淀粉酶的菌株Pseudoalteromonas sp.AN175。生长发酵单因素试验表明,菌株AN175在1%麦芽糖、0.5%酵母粉、0.01%磷酸铁、0.5%氯化钙、1%可溶性淀粉、5%NaCl、pH 6.0的培养基,10℃,30%装液量,130 r/min条件下振荡培养9 d可达到最大酶活132.5 U/mL,为优化前的2倍多。生长和产酶试验表明,该菌生长较常温菌缓慢,酶分泌时间滞后。粗酶特性研究显示,该酶催化的最适条件为温度30℃,pH值8.0。The antarctic strain Pseucloalterornonas sp. AN175 was screened from 147 sea-ice bacteria which could produce low-temperature α-amylase. Through single factor method experiment, the enzyme-producing conditions were 1% maltose, 0.5% yeast powder, 0.01% Fe3PO4, 0.5% CaCl2, 1% soluble starch, 5% NaC1, pH6.0, at 10℃, 130 r/min shaking bed cultivated for 9 days. Under optimal cultivate conditions, a-amylase activity was improved from 64.5 U/mL to 132.5 U/mL. Comparing to temperature bacteria strain, sea-ice strain AN175 grew and produced enzyme slower. Preliminary studies showed that the most adaptable reaction temperature and pH for α-amylase were 30 ℃ and 8.0 respectively.

关 键 词：南极细菌 低温α-淀粉酶 筛选 发酵条件 酶学特性 

分 类 号：TQ925.1[轻工技术与工程—发酵工程] P462.41[天文地球—大气科学及气象学]