多胺柔性链改性壳聚糖微球固定化木瓜蛋白酶  

Immobilization of papain on flexible polyethylenamine chain modified chitosan microspheres

作　　者：王红艳[1] 朱建星[1] 张万忠[1] 杨颖[1] 

机构地区：[1]沈阳化工学院环境与生物工程学院,辽宁沈阳110142

出　　处：《食品工业科技》2010年第3期267-270,共4页Science and Technology of Food Industry

基　　金：辽宁省教育厅科学研究计划资助(05L344)

摘　　要：用反相悬浮交联法制备了单分散壳聚糖微球树脂,以其作为固定化载体基体,经氨基保护、C6羟基环氧化后接枝亲水性多乙烯多胺,制备了粒径为220～300μm、具有较高机械强度的多胺柔性链改性壳聚糖载体。采用该载体对木瓜蛋白酶在pH8.0缓冲液中室温下固定25h,固定化酶表观活力最高可达313U.g-1,活力回收率最高达61.5%,是采用未经多胺分子修饰的壳聚糖微球固定化的2.3倍。该柔性固定化酶的最适温度为65℃,最适pH为8.0,连续使用6～7次后仍保持原来活力的一半。Monodisperse chitosan microspheres in the immobilized enzyme by phase-inversion suspension range 220μm to 300μm were prepared as support matrix for cross-linking technique firstly,then flexible polyethylenamine chain modified chitosan microspheres with more amino group content and better mechanical resistance were prepared by reaction of polyethyleneamine and epoxy-activated chitosan.Papain was immobilized on the support. The apparent activity of the immobilized Papain reached 313U/g under those immobilization conditions： pH 8.0, and reaction time 25h at room temperature.The most recovery of immobilized Papain activity reached 61.5% ,which was about 2.3 times that as immobilization on pure chitosan microsphere.The properties of the immobilized papain were also studied.The best application temperature and pH were 65℃ and 8.0 respectively.When used continuously for 6-7 times,the activity of the immobilized papain would fall by nearly half.

关 键 词：壳聚糖 多乙烯多胺 固定化载体 微球 木瓜蛋白酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]