Cytotoxicity of lambda-cyhalothrin on the macrophage cell line RAW 264.7  

作　　者：Quan Zhang, Cui Wang, Liwei Sun, Ling Li, Meirong Zhao Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. 

出　　处：《Journal of Environmental Sciences》2010年第3期428-432,共5页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundations of China (No. 20877071, 20837002);the National Basic Research Program (973) of China (No.2009CB421603)

摘　　要：The wide use and wide-spectrum toxicity of synthetic pyrethroids （SPs） insecticides make them an emerging ecotoxicological concern. Some previous studies showed that SPs possessed cytotoxicity in some immune cells such as human lymphocytes and rat bone marrow. However, the cytotoxicity of SPs to macrophages, which are crucial to innate immunity, has not been explored. In the present report, we investigated a new pyrethroid insecticide, lambda-cyhalothrin （LCT）, which may increase the generation of reactive oxygen species （ROS） and DNA damage levels and cause cytotoxicity in RAW 264.7 cells in dose- and time-dependent manners. The results for the first time implicated increased endogenous ROS and DNA damage as co-mediators of LCT-induced cytotoxicity in macrophages. Our results also suggested that macrophages were involved in synthetic pyrethroid-induced adverse immune effects. Considering the ubiquitous environmental presence of SPs, this study provided new information relative to the potential long-term physiological and immunological effects associated with chronic exposure to SPs. Hence, the potential immunotoxicity of SPs should be considered in assessing the safety of these compounds in sensitive environmental compartments.The wide use and wide-spectrum toxicity of synthetic pyrethroids （SPs） insecticides make them an emerging ecotoxicological concern. Some previous studies showed that SPs possessed cytotoxicity in some immune cells such as human lymphocytes and rat bone marrow. However, the cytotoxicity of SPs to macrophages, which are crucial to innate immunity, has not been explored. In the present report, we investigated a new pyrethroid insecticide, lambda-cyhalothrin （LCT）, which may increase the generation of reactive oxygen species （ROS） and DNA damage levels and cause cytotoxicity in RAW 264.7 cells in dose- and time-dependent manners. The results for the first time implicated increased endogenous ROS and DNA damage as co-mediators of LCT-induced cytotoxicity in macrophages. Our results also suggested that macrophages were involved in synthetic pyrethroid-induced adverse immune effects. Considering the ubiquitous environmental presence of SPs, this study provided new information relative to the potential long-term physiological and immunological effects associated with chronic exposure to SPs. Hence, the potential immunotoxicity of SPs should be considered in assessing the safety of these compounds in sensitive environmental compartments.

关 键 词：LAMBDA-CYHALOTHRIN MACROPHAGE reactive oxygen species CYTOTOXICITY 

分 类 号：X171.5[环境科学与工程—环境科学]