膜生物反应器用于乙醇连续发酵菌株的适应性培养  

Yeast acclimatization in a silicone rubber membrane bioreactor for ethanol continuous fermentation

作　　者：傅晟伟[1] 汤晓玉[1] 周一慧[1] 蒋越[1] 陈春燕[1] 肖泽仪[1] 

机构地区：[1]四川大学化学工程学院,四川成都610065

出　　处：《中国酿造》2010年第3期102-105,共4页China Brewing

基　　金：国家自然科学基金资助项目(20776088)

摘　　要：将一种耐高温酿酒干酵母在体积为5L、膜面积为0.08m2的硅橡胶膜生物反应器乙醇连续发酵系统中进行适应性培养,选育出适应该环境的优势菌种。三轮乙醇连续发酵实验的结果表明,糖的转化率差别不大,但单位酵母细胞代谢产生乙醇的能力有了提高,说明酵母细胞在长期封闭循环发酵环境条件下,原种菌经过几百代繁殖后其后代遗传特征发生了改变,能逐渐适应所处的不利环境。表明通过膜生物反应器长期封闭循环发酵对酵母细胞进行适应性培养,可以获得具有某种遗传优势的新菌株。An alcohol active dry yeast was acclimatized in a silicone rubber membrane bioreactor with effective volume of 5 L and membrane area of 0.08 m^2, and a preferential strain was screened after three rounds of continuous fermentation experiments in this bioreactor. The results of second and the third fermentation showed that there was little difference in the ratio of glucose conversion, however, the ethanol yield of yeast was improved slightly. This means that the genetic character of the offspring had been changed after hundreds of generations in a PDMS membrane bioreactor system, and the strain could gradually adapt the environment. It was showed the strain screened by this way had some dominant features.

关 键 词：膜生物反应器 酵母 适应性培养 

分 类 号：TQ920.5[轻工技术与工程—发酵工程]