海洋细菌B1109产胞外多糖的培养条件  

Exopolysacchrides production by marine bacterium B1109

作　　者：张熹[1] 祖国仁[1] 陈莉[1] 刘阳[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2010年第2期101-105,共5页Journal of Dalian Polytechnic University

摘　　要：生物多糖是在食品、化工与医药领域有着广泛用途的一类高分子生物物质。为了考察海洋细菌B1109产胞外多糖培养的条件,对从海泥中分离到的1株海洋细菌B1109产胞外多糖的碳源、氮源、碳氮浓度等营养元素和培养基初始pH值、培养基装量、接种量、培养温度等培养条件进行了研究。该菌株产胞外多糖最佳碳源为葡萄糖,最佳氮源为硫酸铵,质量浓度分别为20和4 g/L;最佳培养条件为:培养基初始pH 8.0,培养基装量400 mL/L,接种量6%,培养温度24℃。在此条件下培养120 h,海洋细菌B1109产胞外多糖4.29 g/L。Exopolysaccharides-producing strain Bl109 was isolated from oozed sea water and the culture conditions were investigated. The optimal culture conditions for exopolysaccharides production were as follows： 20 g/L glucose, 4 g/L ammonium sulfate, the initial pH 8.0, the loading sample 400 mL/L, the inoculum 6% and the temperature 24℃. When cultured under such conditions for 120 h, the strain B1109 could produce 4.29 g/L exopolysaccharids.

关 键 词：海洋细菌 胞外多糖 海洋生物多糖 

分 类 号：TS201.25[轻工技术与工程—食品科学] Q813.11[轻工技术与工程—食品科学与工程]