正交试验法优化黑米黑色素的超声辅助提取工艺  

Orthogonal Array Design Tests for Optimizing Ultrasound-assisted Extraction of Black Rice Pigment

作　　者：张吉祥[1] 赵文静[1] 白晓杰[1] 欧来良[2] 

机构地区：[1]廊坊师范学院化学与材料科学学院,河北廊坊065000 [2]南开大学分子生物学研究所生物活性材料教育部重点实验室,天津300071

出　　处：《食品科学》2010年第4期39-41,共3页Food Science

基　　金：河北省教育厅基金资助项目(2007317);廊坊师范学院科学基金资助项目(LSZB200801)

摘　　要：以黑米为原料,优化黑色素的最佳超声辅助提取工艺。采用正交试验法,研究超声波功率、浸提时间、浸提剂体积分数及料液比(黑米︰乙醇溶液,m/V)对黑米色素提取率的影响。结果表明:在所考察的因素中,黑米黑色素的最佳提取工艺为A3B1C3D3。浸提黑色素宜选体积分数80%的乙醇溶液作为提取剂,超声波作用时间50min,料液比1:32,超声功率250W,该优化方法的提取率为4.5%,比传统的索式提取法的提取率提高了近3倍。Ethanol was used for extracting black rice pigment under ultrasound assistance.To maximize the extraction of black rice pigment,orthogonal array design was employed for investigating the effects of ethanol concentration,solid-to-liquid ratio,ultrasound power,extraction time on black rice pigment yield （represented by the absorbance at 543 nm wavelength of extract solution）.Results showed that the use of 80% ethanol for extracting black rice grains at 1：32 solid-to-liquid ratio （m/V） with the assistance of 50 min ultrasound treatment at 250 W gave an optimal pigment extraction and the resulting pigment yield reached up to 4.5%,with a near 3-fold increase in comparison with the conventional Soxhlet extraction.

关 键 词：黑米 黑色素 正交试验法 超声提取 

分 类 号：TS202.3[轻工技术与工程—食品科学]