基因重组木聚糖酶PTX2高密度发酵制备及其功效研究  

High-density Fermentation and Functional Characterization of Recombinant Xylanase PTX2

作　　者：尹佳[1] 王利[1] 何军[1] 余冰[1] 陈代文[1] 

机构地区：[1]四川农业大学动物营养研究所,教育部动物抗病营养重点开放实验室,四川雅安625014

出　　处：《食品科学》2010年第5期165-168,共4页Food Science

基　　金：四川省科技攻关项目(2007Z06-050);教育部"长江学者和创新团队发展计划"项目(IRT0555-5);四川省教育厅青年基金项目(00924200)

摘　　要：利用15L液体发酵系统对基因重组木聚糖酶PTX2进行高密度发酵制备,结果显示,在搅拌速度450r/min,甲醇流速6mL/(L·h)条件下,连续诱导96h后,其最大菌体质量浓度为78g/L,最高酶活力达560U/mL;在断奶仔猪饲粮中添加500U/kg未经任何处理的液体基因重组木聚糖酶PTX2,仔猪平均日增体质量(ADG)提高16.9%,料与体质量比显著降低,饲粮粗蛋白、灰分、钙及粗纤维表观消化率也有增加的趋势。该研究结果初步证实了重组木聚糖酶PTX2在饲料及食品工业中具有较大的应用价值和开发前景。Recombinant xylanase PTX2 was prepared in a 15 L fermentor through high-density fermentation. After methanol induction at 28 ℃ for 96 h, the highest cell density and the maximal xylanase activity were achieved to 78 g/L and 560 U/mL, respectively. Diet supplemented with 500 U/kg of unprocessed PTX2 could result in an increase of average body weight gain of weaned piglets by 16.9%, an obvious decrease in intake/body weight gain ratio, and an increasing trend of apparent digestibility of crude protein （CP）, ash, calcium and crude fiber （CF）. Therefore, the potential benefit of PTX2 in feeding weaned piglets demonstrates an attractive application value and industrial development of this enzyme.

关 键 词：重组木聚糖酶 高密度发酵 断奶仔猪 生长性能 表观消化率 

分 类 号：Q81[生物学—生物工程]