牛蒡菊糖和菊芋菊糖对酒精诱导大鼠慢性氧化损伤的防治作用  

Protective Effect of Burdock Inulin and Jerusalem Artichoke Inulin against Chronic Alcohol-induced Oxidative Injury in Mice

作　　者：鲁政[1] 张静[1] 高兆兰[1] 张波[1] 徐永杰[2] 

机构地区：[1]北京联合大学应用文理学院生物学系,北京100191 [2]首都师范大学生命科学学院,北京100048

出　　处：《食品科学》2010年第5期270-273,共4页Food Science

基　　金：北京市教委科技发展项目(KM200711417001)

摘　　要：目的:研究酒精对小鼠肾、脑、心脏及睾丸中GSH、MDA的影响,并探讨牛蒡菊糖在保护脏器氧化损伤方面的作用并与菊芋菊糖进行比较研究。方法:雄性成年小鼠随机分为6组(牛蒡菊糖低、中、高3个剂量组,菊芋菊糖组,酒精模型组和空白对照组),共灌胃20d。实验结束后,分别取脑、肾、心脏和睾丸,制成10%组织匀浆,离心,取上清液测定丙二醛(MDA)、还原性谷胱甘肽(GSH)含量。结果:1)肾脏:小鼠灌胃酒精后,GSH含量与对照组比较差异不显著(P>0.05),灌胃酒精和不同剂量牛蒡菊糖以及菊芋菊糖后GSH含量显著低于酒精组(P<0.05,P<0.01);小鼠灌胃酒精后MDA含量与对照组比较显著升高(P<0.01),灌胃酒精和中高剂量牛蒡菊糖后MDA含量显著低于酒精组(P<0.05),但灌胃酒精和菊芋菊糖后MDA含量与酒精组比较差异不显著(P>0.05)。2)脑:小鼠灌胃酒精后GSH含量与对照组比较显著降低(P<0.01),灌胃酒精和中高剂量牛蒡菊糖后GSH含量显著高于酒精组(P<0.01),但灌胃酒精和菊糖后GSH含量与酒精组比较无显著性差异(P>0.05);小鼠灌胃酒精后MDA含量与对照组比较显著升高(P<0.01),灌胃酒精和高剂量牛蒡菊糖以及菊芋菊糖后MDA含量与酒精组比较显著降低(P<0.01)。3)心脏:小鼠灌胃酒精后GSH含量与对照组比较略有降低但无显著性差异(P>0.05),灌胃酒精和高剂量牛蒡菊糖后GSH含量显著高于酒精组(P<0.01),但灌胃酒精和菊芋菊糖后GSH含量与酒精组比较无显著性差异(P>0.05);小鼠灌胃酒精后MDA含量与对照组比较无显著差异(P>0.05),灌胃酒精和高剂量牛蒡菊糖以及菊芋菊糖后MDA含量与酒精组比较也无显著差异(P>0.05)。4)睾丸:小鼠灌胃酒精后GSH含量与对照组比较无显著性差异(P>0.05),灌胃酒精和牛蒡菊糖以及菊芋菊糖后GSH含量与酒精组比较变化也无显著性差异(P>0.05);小鼠灌胃酒精后MDA含量与对照组比较略有升高但无显著差异(PObjective： To investigate and compare the protective effects of burdock inulin and Jerusalem artichoke inulin against oxidative damage of organs in mice based on the examinations of body weight, viscera coefficients, and GSH and MDA contents of kidney, brain, heart and testis. Methods： Male mice were randomly divided into 6 groups, which were designated as Burdock inulin groups at low, middle and high dosages, Jerusalem artichoke inulin group, alcohol model group and blank control group to perform the corresponding treatments for 20 consecutive days. At the end of administration, all mice were sacrificed and anatomized to harvest kidney, brain, heart and testis for measuring the contents of GSH and MDA. Results： Compared with the blank control group, no obvious differences of GSH content in kidney, heart and testis and a significant difference of GSH content in brain of the alcohol model group were observed. Meanwhile, the alcohol model group exhibited an obvious increase of MDA content in kidney and brain and no obvious difference of MDA content in heart and testis. However, compared with the alcohol model group, the GSH content exhibited a significant decrease in kidney of the mice treated with burdock inulin at different dosages and in brain of the mice treated with burdock inulin at middle and high dosages; whereas, the GSH content exhibited an obvious difference in heart and testis of the mice treated with burdock inulin. Moreover, the MDA content exhibited a significant decrease in kidney of the mice treated with burdock inulin at middle and high dosages, in brain of the mice treated with burdock inulin at high dosage and in testis of the mice treated with Burdock inulin at high dosage; whereas, the MDA content exhibited an obvious difference in heart of the mice treated with burdock inulin. Conclusion： alcohol can result in significantly oxidative damage of kidney and brain tissue in mice, but exhibit less damage to heart and testis. Burdock inulin and Jerusalem artichoke inulin both exhibit a prot

关 键 词：牛蒡菊糖 酒精 小鼠 器官 氧化损伤 

分 类 号：R151.2[医药卫生—营养与食品卫生学]