毛细管气相色谱法测定含奶油类冰淇淋等样品中甜蜜素  

Determination of Sodium Cyclamate in Ice Cream Containing Cream with Capillary Gas Chromatography

作　　者：张霞[1] 王锡宁[1] 张萍[1] 

机构地区：[1]山东省疾病预防控制中心,山东济南250014

出　　处：《预防医学论坛》2010年第3期248-250,共3页Preventive Medicine Tribune

摘　　要：[目的]探讨含奶油类冰淇淋等样品中甜蜜素的前处理及提高测定灵敏度的方法。[方法]液体样品前处理加亚铁氰化钾及乙酸锌沉降蛋白，处理液用毛细管色谱柱程序升温检测。[结果]在0．01～10mg范围内，甜蜜素测定的线性相关系数r=0．999。若取10g样品测定，其定量检出限为0．005g／kg，高、中、低三种浓度的加标回收率分别在89．0％-103．8％．[结论]该方法适用于含奶油类冰淇淋等样品中甜蜜素的测定。[Objective] To explore a method in pre-treatment of sodium cyclamate of ice cream samples containing cream,and to improve the determination sensitivity. [Methods]Pre-treatment of the liquid sample was conducted with potassium ferrocyanide and zinc acetate to sediment protein, The treating liquid was tested with temperature-programmed capillary column. [Results]The linear correlation coefficient of sodium cyclamate determination was r = 0, 999 in the range of 0.01-10 mg. If the tested sample was 10 g,the quantitative detection limit was 0. 005 g/kg. The addition standard recovery with high,medium and low concentration was from 89.0% to 103.8%.[Conclusion]The method is suitable for the determination of sodium cyclamate of ice cream samples containing cream.

关 键 词：毛细管气相色谱 冰淇淋 甜蜜素 

分 类 号：R155.57[医药卫生—营养与食品卫生学]