1株产几丁质酶绿僵菌的筛选及其产酶特性  

Screening of Metarhizium anisopliae Produced Chitinase and Its Enzyme-Producing Characteristic

作　　者：王子坚[1,2] 曾巧英[1] 殷宏[2] 刘志杰[2] 任巧云[2] 罗建勋[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《贵州农业科学》2010年第3期87-90,共4页Guizhou Agricultural Sciences

基　　金：国家支撑计划项目"主要动物源性人畜共患细菌和寄生虫病防控生物新制剂与开发"(2007BAD40B06);国家高技术研究发展计划(863计划)重点项目"硬蜱基因工程疫苗的研究"(2006AA10A207)

摘　　要：为了提高绿僵菌产几丁质酶的酶活力,在自然条件下,从患病蜱体表分离到10株绿僵菌与8株白僵菌,通过筛选,得到1株绿僵菌Ma002。对该菌液体深层发酵产几丁质酶进行了测定,结果表明:几丁质酶的最佳产酶条件:碳源为胶体几丁质,氮源为蛋白胨,接种种龄为24h,以瓶体积的12%为装基量,在温度26℃,起始pH值7.5～8.0,搅拌速度180r/min,产酶时程96h,酶活力可达2.63U/mL,比原基础培养基上的酶活力(1.67U/mL)提高57%。10 isolates of Metarhizium anisopliae and 8 isolates of Beauveria bassiana were isolated from dead body of tick in nature conditions to increase the chitinase-producing ability of M. anisopliae. One strain of M. anisopliae Ma002 was screened out. The chitinase produced from submerged fermentation of this strain was determined. The results showed that the optimal fermentation conditions for enzyme-producing were as following： colloid chitin as carbon source, peptone as nitrogen source, age to be inoculated 24 h, optimized volume of cultural medial in flask with 12%,pH 7. 5-8. 0 at 26℃, enzyme-producing cultured for 96 h on 180 r/min shaker. The chitinase activity could reach 2.63 U/mL in above conditions. It increased 57% comparing with the original basic medium.

关 键 词：几丁质酶 酶活力 发酵 

分 类 号：Q55[生物学—生物化学] TQ92[轻工技术与工程—发酵工程]