一种新型支架在乳酸发酵中的应用  

Production of L-lactic acid with immobilized cells of Rhizopus oryzae on a novel matrix

作　　者：王润光[1] 王远亮[1] 王珍[1] 高文娟[1] 任慧卿[1] 刘钊[1] 张晓喜[1] 邱敏[1] 

机构地区：[1]生物流变科学与技术教育部重点实验室,重庆大学生物工程学院,重庆400030

出　　处：《化工进展》2010年第4期733-738,共6页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(30870609);重庆市自然科学基金重点项目(CSTC2009BA4025)资助

摘　　要：采用一种新型载体支架来进行米根霉的固定化发酵研究。种子培养过程中,5h后孢子已全部吸附到支架上,种子培养时间为24h,生物量维持在6.8g/L以上;发酵过程中,在温度为33℃,摇床转速为180r/min,装液量为20%,支架数为3个,时间为48h,发酵过程中及时补加CaCO3的条件下,L(+)-乳酸平均浓度可达50.00g/L,转化率接近65.0%,产率可保持在1.0g/(L·h)以上。与游离发酵相比,固定化发酵乳酸浓度、转化率、产率分别提高了79%、250%和150%,发酵周期提前了24h,且能重复发酵7个批次。A novel matrix was developed for fermentation to produce L-lactic acid by Rhizopus oryzae at shaker-level.Fungal mycelia were immobilized on the matrices to study the fermentation conditions and process stability.During the seed culture process,all the spores were adsorbed after only 5 h,biomass concentration was up to 6.8g/L after 24 h.Under optimized conditions using three matrices at 33 ℃ and 180 r/min with volume ratio of 20% by adding CaCO3 in time,L-lactic acid was obtained after 48 h fermentation with concentration of 50.00 g/L in 65.0% yield and the productivity was 1.0 g/（L·h）.Compared with cell-free fermentation,the concentration of L-lactic acid,the yield and the productivity increased by 79%,250% and 150%,respectively,and the fermentation period decreased by 24 h.The process of immobilized fermentation showed good stability in seven repeated batches.

关 键 词：L(+)-乳酸 米根霉 固定化发酵 支架 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]