嗜热枯草芽孢杆菌TT68产β-甘露聚糖酶发酵条件的优化  

Optimization of β-mannanase Production by the Thermophilic Bacillus subtilis

作　　者：唐荦[1] 唐艳斌[1] 江正强[1] 周鹏[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院

出　　处：《食品与发酵工业》2010年第3期15-19,共5页Food and Fermentation Industries

基　　金：国家"863"计划课题(No.2006AA10Z337)部分内容

摘　　要：研究了嗜热枯草芽孢杆菌TT68的发酵条件的优化。经碳源、氮源、碳氮比、氨基酸和添加维生素5个单因素发酵条件的优化,得到枯草芽孢杆菌TT68发酵产β-甘露聚糖酶的最适摇瓶发酵培养条件:4g/L魔芋精粉为碳源,0.7g/L豆粕粉为氮源,碳氮比为6∶1,2g/LL-缬氨酸和0.75mg/L盐酸吡哆辛。在上述条件下培养6d,胞外β-甘露聚糖酶活性最高,可达7500U/mL。采用5L发酵罐进行放大培养,48h酶活即达到最高,可达9870U/mL。该嗜热枯草芽孢杆菌能利用廉价的碳源和氮源高产甘露聚糖酶,且发酵周期短,在工业化生产甘露聚糖酶中具有很大的应用潜力。The thermophilic Bacillus subtilis TT68 was optimized for β-mannanase production in the present report. The fermentation conditions, including carbon source, nitrogen source, ratio of carbon source and nitrogen source, amino acids and vitamins, were optimized in order to improve the production of β-mannanase. The optimized medium is composed of 4 g/L konjac powder as carbon source, 0.7 g/L soybean meal powder, 2 g/L L-valine, and 0.75 mg/L Pyridoxine hydrochloride （VB6）. The highest enzyme activity of 7500 U/mL was obtained after incubation for 6 days in the optimized medium. When the strain was incubated using a 5 L fermentor for 2 days, the activity was up to 9870 U/ mL. Bacillus subtilis TT 68 could produce a high yield of β-mannanase utilizing the inexpensive carbon source and nitrogen source in a short period. Thus, the strain displays a great potential for the industrial production of β-mannanase.

关 键 词：嗜热枯草芽孢杆菌 Β-甘露聚糖酶 发酵 魔芋精粉 

分 类 号：TQ925[轻工技术与工程—发酵工程]