耐高温酸性脂肪酶菌株NJY-1-3的选育及发酵条件的研究  

A research on screening and fermentation conditions of thermostable Lipase strain NJY-I-3

作　　者：闫丽娟[1,2,3] 谢振荣[1,2,3] 赵春雷[1,2,3] 高润池[1,2,3] 李俊[1,2,3] 唐湘华[1,2,3] 黄遵锡[1,2,3] 

机构地区：[1]生物能源持续开发利用教育部工程研究中心,昆明650092 [2]云南师范大学酶工程重点实验室,昆明650092 [3]云南师范大学生命科学学院,昆明650092

出　　处：《食品科技》2010年第3期11-15,共5页Food Science and Technology

基　　金：国家863计划项目(2008AA02Z202)

摘　　要：从云南省福贡县的玉米地土壤样品中筛选到一株耐高温产酸性脂肪酶的菌株NJY-1-3,通16SrDNA鉴定该菌株为伯克氏菌(Burkholderia pseudomallei)。对其发酵条件进行研究结果表明:该菌株产酶的最适培养时间为72h,酶促反应的最适作用pH4.0,最适作用温度为65℃,摇瓶发酵最适产酶条件为:橄榄油1%,MgSO4·7H2O0.05%,K2HPO40.1%,酵母膏1.0%,蔗糖0.8%,60mL单蒸水。在此条件下发酵脂肪酶酶活力可达到50.50IU/mL。该酶学特性非常适用于食物的处理、医药工业及生物柴油的制备等。AA acidic thermostable Lipase strain NJY-1-3 was isolated from soil of the corn field Nujiang Yunan.The strain was identified as Burkholderia pseudomallei.by the methods of 16S rDNA of bacteria.The experiment is a tentative research on the fermentation conditions and enzymatic properties with the object of the acidophilic strain NJY-1-3 with the production of Lipase.The enzyme have optimum time of cultivate at 72 h,with a most proper temperature of 65 ℃,and a most proper pH of 4.0.The best ingredient of the bacteria culture medium is：olive oil 1%,MgSO4·7H2O 0.05%,K2HPO4 0.1%,yeast 1.0%,cane sugar 0.8%,60 mL ddH2O.In this condition,the lipase activity reached 50.50 IU/mL.It can use in medical applications,bioremediation of food,production of Biodiesel and so on.

关 键 词：脂肪酶 耐高温 菌种选育 发酵条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]