碱性脱毛蛋白酶产生菌的选育  

Isolation of the alkaline Unhairing Protease Producing Strain

作　　者：刘彦[1,2] 何先祺[1,2] 张义正[1,2] 

机构地区：[1]四川联大皮革系 [2]四川联大生工系

出　　处：《皮革科学与工程》1998年第4期6-13,共8页Leather Science and Engineering

摘　　要：从富含蛋白质的土壤中筛选出的碱性蛋白酶产生菌，命名为Ｂａｃｉｌｕｓｓｐ．８（简称为Ｎｏ．８）。Ｎｏ．８菌能在ｐＨ６－１２且含有较高浓度钙离子条件下生长并产酶，与目前的碱性蛋白酶生产菌如地衣芽孢杆菌２７０９相比，它具有较强的耐碱性和耐钙离子性。培养基起始ｐＨ值为７．５，接种菌龄为１８ｈ种子液２％。在３５～３７℃，培养４８～６０ｈ的条件下，Ｎｏ菌产生的蛋白酶活力可稳定在１７００～１８００ｕ／ｍｌ，并且在浸灰过程中可以脱毛。酶的最适ｐＨ为９．６，ｐＨ１０．６－１１．６最稳定。最适温度为４０℃。A alkaline unhairing protease producing strain, named Bacillus sp.8(simply No.8) has been isolated from rich protein-containing soil for the first time. Fermentation condition of the strain has been studied. The results showed that soy bean cake meal and sweet potato meal(maize meal or glucose) are the best nitrogen and carbon sources for the isolated strain to produce proteases. The optimum conditions for enzyme production were:initial pH7.5, 35-37℃for 48-60h. 0.4-0.6% Na 2HPO 4 had a positive effect on the enzyme production. Strain No8 produced maximum alkaline protease activity 1800u/ml. The optimum pH and temperature for the protease activity production are pH 9.6,40℃. The enzyme were stable over pH 10.6. Comparing with the alkaline producing producer B.licheniformis 2709, the isolated strain has higher enduring of alkaline and Ca 2+ .

关 键 词：碱性蛋白酶 脱毛 筛选 菌株选育 制革 

分 类 号：TS541[轻工技术与工程—皮革化学与工程] TQ925.2[轻工技术与工程—发酵工程]