利用表面等离子共振技术快速检测牛奶中的磺胺甲噁唑  

Surface Plasmon Resonance for Rapid Determination of Sulfamethoxazole in Milk

作　　者：周宏敏[1] 欧惠超[1] 姜浩[1] 江海峰[1] 王晓萍[2] 罗昭锋[1] 

机构地区：[1]合肥微尺度物质科学国家实验室,中国科学技术大学生命科学学院,安徽合肥230027 [2]浙江大学光电信息工程学系,浙江杭州310027

出　　处：《食品科学》2010年第6期168-171,共4页Food Science

基　　金：国家"863"计划项目(2006AA06Z406;2007AA09Z114);国家自然科学基金项目(30727001);浙江省科技计划优先课题(2007C13066)

摘　　要：利用表面等离子共振(SPR)技术,建立快速定量检测牛奶中磺胺甲噁唑(SMX)的方法。将SMX共价偶联到表面等离子共振芯片表面,并对抗体的结合浓度及芯片的再生条件进行优化,检测芯片的稳定性。在无抗牛奶中添加系列质量浓度的SMX,利用免疫竞争抑制原理构建标准曲线,并对市售18个牛奶样品进行检测。结果表明:制备的芯片稳定,90个循环相对标准偏差(RSD)为1.23%;该方法的检测限为3.2ng/mL;18个牛奶产品中16个SMX的残留量在规定的允许范围内。所建立的方法可以在15min内完成样品的前处理和检测,是一种简便快捷的定量检测方法。A method based on surface plasmon resonance technique was developed for rapid determination of sulfamethoxazole（SMX） in milk.SMX was covalently coupled to laboratory prepared CM5 chip to obtain SMX chip.The optimal antibody working concentration and chip regeneration conditions were investigated.Meanwhile,the stability of SMX chip was measured.A series of different concentrations of SMX was added to blank milk samples to construct a standard curve for SMX quantification according to the principle of competitive immune inhibition assay.Eighteen commercial milk samples were determined by the developed method.SMX chip displayed good stability,and the relative standard deviation（RSD） for 90 cycles was 1.23%.The limit of detection（LOD） of SMX was 3.2 ng/mL.SMX contents below the required threshold level were observed in 16 of 18 milk samples.Less than 15 min was consumed for the determination of SMX by the developed method.Thus,this method may offer a simple and efficient approach for SMX quantification.

关 键 词：表面等离子共振 芯片 磺胺甲噁唑 

分 类 号：TS207.3[轻工技术与工程—食品科学]