Alcalase蛋白酶水解大豆分离蛋白的研究  

Study on hydrolysis of soybean protein isolate by alcalase

作　　者：黄建韶[1] 张洪[1] 赵东海[1] 

机构地区：[1]湖南文理学院生命科学学院,湖南常德415000

出　　处：《湖南文理学院学报（自然科学版）》2010年第1期28-30,共3页Journal of Hunan University of Arts and Science(Science and Technology)

摘　　要：研究了Alcalase蛋白酶对大豆分离蛋白的水解作用,分析了pH、温度、酶浓度、底物浓度和水解时间对大豆分离蛋白酶解的影响,通过单因素分析、正交试验,确定了Alcalase蛋白酶水解大豆分离蛋白的最佳水解条件,即pH8.0,温度60℃,酶浓度1000U/g,底物浓度3%,水解时间2h.Hydrolysis process for soybeen protein isolate with Alcalase was studied. And the effects of the main parameters including pH, hydrolysis temperature, enzyme concentration, substrate concentration, hydrolysis time on hydrolysis reaction were investigated. The optimum conditions had been found by mono-factor analysis and orthogonal test as follows, pH： 8.0, hydrolysing temperature： 60℃, enzyme concentration： 1 000 u per gram of substrate, substrate concentration： 3%, hydrolysis time： 2 h.

关 键 词：蛋白酶 大豆分离蛋白 酶解 

分 类 号：Q556.9[生物学—生物化学]