纳豆激酶分离纯化方法的研究  

Method of separation and purification of nattokinase

作　　者：史延茂[1] 马跃华[1,2] 杨明[1,2] 董超[1] 

机构地区：[1]河北省生物研究所,河北石家庄050081 [2]河北工业大学化工学院,天津300130

出　　处：《中国酿造》2010年第4期119-123,共5页China Brewing

摘　　要：采用硫酸铵二级盐析、330（OH）型树脂脱色、CM-52阳离子树脂离子交换层析三步法从发酵液中提取纳豆激酶。结果发现：（1）硫酸铵盐析采用20％～60％饱和度范围，纯化倍数为3．23，收率为72．36％；（2）330（0H）型树脂脱色，在脱色的同时可以除去杂蛋白，样品纯度达到电泳显示2条带：（3）CM．52阳离子交换树脂对纳豆激酶属于优吸型吸附，在pH6．0，0．01mol／L的PBS中交换效果最好，可以得到电泳纯的纳豆激酶，总收率为48．51％。The extraction methods of nattokinase from fermentation fluid were studied with two degree saltout of （NH4）2SO4, decolor of resin 330 （OH） and positiveion resin CM-52 Ion exchange. As a result： （1） the saturation of （NH4）2SO4 saltingout was 20 %-60 %, purification multiple is 3.23, recovery was 72.36 %; （2） after resin 330 （OH） decolorization, the sample can achieve the purity of electrophoresis and demonstrate two belts; （3） positive ion resin CM-52 ion exchange adsorption was superior adsorption of nattokinase, the best exchange effect was with pH 6.0, 0.01 mol/L PBS, existing only one elution peak, it can dissolve the hitch and its purity of electrophoresis of nattokinase. The total recovery was 48.51%.

关 键 词：纳豆激酶 分离纯化 盐析 脱色 离子交换 

分 类 号：TQ925[轻工技术与工程—发酵工程]