大蒜生理活性物质对胃癌细胞和黑色素瘤细胞抑制作用的研究  

Inhibitory effect of physiological active substances from garlic on gastric cancer cell and melanoma cell

作　　者：王霞[1] 苟萍[1] 孙桂琳[1] 孙素荣[1,2] 索菲娅[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]新疆生物资源基因工程重点实验室 新疆大学,新疆乌鲁木齐830046

出　　处：《中成药》2010年第4期557-561,共5页Chinese Traditional Patent Medicine

基　　金：新疆生物资源基因工程重点实验室开放项目(XJDX0201-2009-08)

摘　　要：目的:研究大蒜生理活性物质对人胃癌细胞MGC-9和小鼠黑色素瘤细胞B16细胞增殖及细胞周期的影响。方法:用倒置显微镜观察细胞形态,噻唑蓝(MTT)比色法测定细胞增殖抑制率;流式细胞仪检测B16细胞周期分布的影响。结果:经大蒜素及其前体物质蒜氨酸、蒜氨酸酶和蒜氨酸+蒜氨酸酶处理后,MGC-9和B16细胞不同程度变圆,体积缩小,脱壁细胞增多;除蒜氨酸酶外,蒜氨酸、大蒜素和蒜氨酸+蒜氨酸酶都能诱导MGC-9和B16产生时间和剂量依赖性的增殖抑制作用,其中蒜氨酸+蒜氨酸酶的作用尤为显著。大蒜素和蒜氨酸+蒜氨酸酶阻滞B16细胞于G0/G1期。结论:本文由大蒜提取物处理后结果与已发表的临床研究相一致。AIM： To study the effect of garlic extracts on cell proliferation and cell cycle on human gastric cancer cell line（MGC-9） and mouse murine melanoma cell line（B16）.METHODS： Cell morphology was ob-served by inverse microscopy.Cytotoxicity was examined by dimethyl thiazolyl tetrazolium bromide assay.Cell cycle distribution was detected by flow cytometry.RESULTS： After being treated with allicin and its precursors-alliin,alliinase and mixture of alliin and alliinase from garlic extracts,cells rounded up,shrank and cell membrane lost its integrity.Allicin,alliin and mixture of alliin and alliinase were all able to exert antiproliferative effects on MGC-9 and B16 cells in a dose-and time-dependent manner,and the inhibitory potency of mixture of alliin and alliinase was more than that of others.Allicin and mixture of alliin and alliinase could arrest the cell cycle of B16 in G0 /G1 phase.CONCLUSION： In experiment the results given by a treatment of garlic extracts are in good agreement with observation established in clinical studies.

关 键 词：大蒜 大蒜素 蒜氨酸 蒜氨酸酶 人胃癌细胞MGC-9 小鼠黑色素瘤细胞B16 细胞增殖 细胞周期 

分 类 号：R285.6[医药卫生—中药学]