功能食品与饮品中牛磺酸的柱前衍生-高效毛细管电泳法快速测定  

Rapid Determination of Taurine in Functional Food and Beverage by High Performance Capillary Electrophoresis with Pre-column Derivatization

作　　者：薛勇[1] 薛丰[1] 孙成均[1] 

机构地区：[1]四川大学华西公共卫生学院卫生检验教研室,四川成都610041

出　　处：《分析测试学报》2010年第4期386-389,393,共5页Journal of Instrumental Analysis

基　　金：国家科技支撑计划资助项目(2006BAD27B02)

摘　　要：建立了功能食品和饮品中牛磺酸的高效毛细管电泳陕速分析方法。以邻苯二甲醛（OPA）为衍生剂进行柱前衍生，采用45mmol／L（pH9．5）的硼砂缓冲溶液为运行缓冲溶液，熔融石英毛细管（50cm×50μm，有效长度41cm，未涂层）为分离柱；柱上紫外睑测波长：335nm，分离电压19kV，电泳温度20℃，重力进样，进样时间10s。牛磺酸的质量浓度在5．0～100．0mg／L范围内呈良好线性关系（r〉0．999），检出限（S／N=3）为1．6mg／L，迁移时问和峰面积的相对标准偏差（n=7）分别为2．4％和3．8％。用该法测定了功能食品、乳制饮品和饮料等样品中的牛磺酸，相对标准偏差为0．7％～3．7％，加标回收率为93％～111％。A new method for the rapid quantification of taurine in functional food and beverage samples by capillary eleetrophoresis with ultraviolet detection was developed. The sample solution was derivatized with o-phthaldialdehyde （OPA） , and separated by high performance capillary electrophoresis （HPCE）. The experimental parameters were as follows： separation column： an uncoated fused-sili- ca capillary（50 cm ×50 μm, and 41 cm effective length） ; running buffer： 45 mmol/L sodium tetraborate（pH 9.5） ; running voltage： 19 kV; temperature： 20 ℃ ; sampling time： 10 s; detection wavelength： 335 nm. The method showed a good linearity within the concentration range of 5.0 - 100. 0 mg/L（ r 〉 0. 999）. The detection limit（ S/N = 3 ） was 1.6 mg/L. The RSDs （ n = 7 ） of migration times and peak areas for taurine standards were 2. 4% and 3.8%, respectively. Satisfactory results were obtained for analysis of functional foods, dairy drinks and beverages with recoveries of 93%- 111% and RSDs of 0. 7% -3.7% .

关 键 词：牛磺酸 高效毛细管电泳法 柱前衍生 功能食品 饮品 

分 类 号：O657.7[理学—分析化学]