纤维素酶活测定影响因素的研究  

Study on impact factors of determination of cellulase activity

作　　者：姜心[1] 陈伟[1] 周波[2] 黄坤勇 韩明渠[2] 

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]山东农业大学生命科学学院,山东泰安271018 [3]天津牧光生物技术有限公司,天津300112

出　　处：《食品工业科技》2010年第5期65-68,共4页Science and Technology of Food Industry

基　　金：国家科技基础条件平台微生物菌种资源整理整合项目(2005DKA21201-13);国家科技支撑计划黄淮平原区农田秸秆资源综合利用技术集成研究与示范(2007BAD89B09-16);现代农业产业技术体系建设专项资金资助;农业部行业科技项目(nyzx07-024);山东省农业重大应用技术创新课题

摘　　要：研究和分析了3,5-二硝基水杨酸法测定羧甲基纤维素酶糖化力的实验条件,尤其是对测定波长的选择、底物和酶液加量、空白样的选择、酶液稀释倍数对酶活的影响等方面进行了创新性的探究;另外对常规性的影响因素:酶促反应时间、反应pH、底物的浓度等传统因素做了研究。实验表明:在40℃,pH4.6,底物浓度为10g/L,底物和酶液加量各2mL,酶促反应时间5min,空白样选择终止空白,测定波长480nm处测定OD值比较适宜。The experimental conditions of determination of carboxymethyl cellulase by the method of 3,5-dinitrosalicylic acid was studied,in particular,determination of wavelength selection,substrate and enzyme solution volume,selection of the blank,enzyme was diluted in multiples of the impact of the activity carried out to explore innovative.In addition,the impact of conventional factors,enzymatic reaction time,reaction pH,substrate concentration were studied.Experiments showed that：40℃,pH4.6,substrate concentration was 10g/L,the volume of enzyme and substrate were 2mL separately,enzymatic reaction time was 5min,select the termination blank as control,and the wavelength at 480nm was much better for determination.

关 键 词：纤维素酶活力 3 5-二硝基水杨酸(DNS) 羧甲基纤维素钠(CMC) 

分 类 号：TS201.25[轻工技术与工程—食品科学]