Kitasatospora sp. MY5-36菌株补料重复发酵生产ε-聚赖氨酸  

Repeated Fermentation with Variable Feeding for Production of ε-Poly-L-lysine by Kitasatospora sp. MY 5-36

作　　者：张扬[1] 张全景[1] 冯小海[1] 徐虹[1] 

机构地区：[1]南京工业大学食品与轻工学院材料化学工程国家重点实验室,江苏南京210009

出　　处：《过程工程学报》2010年第2期344-347,共4页The Chinese Journal of Process Engineering

基　　金：江苏省农业支撑基金资助项目(编号:BE2009363);江苏省博士后基金资助项目(编号:0901011C)

摘　　要：以Kitasatospora sp. MY5-36菌株为研究对象,首次采用带补料的重复发酵工艺发酵生产ε-聚赖氨酸(ε-PL),并对工艺参数进行初步研究.在摇瓶上对重复发酵ε-PL的可行性及稳定性进行了验证,并在5L发酵罐上初步构建了带补料的重复发酵ε-PL工艺.研究结果表明,重复发酵过程中最佳留种量为10%().通过对间歇补料、变速补料2种补料方式的考察,确定当残糖浓度下降至10g/L时,采用变速补料并控制残糖浓度在10g/L时能较好地进行带补料的重复发酵生产ε-PL.经5批次发酵后发酵体系仍具有较高的ε-PL生产能力,ε-PL批次平均终浓度为20g/L,生产强度达到0.159g/(L·h).Repeated fermentation with variable feeding for production of ε-poly-L-lysine（ε-PL） by Kitasatospora sp.MY 5-36 was investigated.It was demonstrated that the repeated fermentation in shaking flask had good feasibility and stability.The ε-PL production was also studied in a 5 L bioreactor.The maximum ε-PL concentration was obtained when 10%（） fermentation broth was left as the seed for the next batch of repeated fermentation.The feeding should be started when the fermentation broth of residual sugar concentration decreased to 10 g/L.During the repeated fermentation,the glucose concentration was maintained at about 10 g/L with variable flow feeding.A high level of ε-PL production capacity still remained after 5 batches of fermentation,with approximate average batch ε-PL concentration of 20.0 g/L under a productivity of 0.159 g/（L·h）.

关 键 词：Ε-聚赖氨酸 Kitasatospora sp. MY5-36 重复发酵 

分 类 号：TQ920[轻工技术与工程—发酵工程]