硒和铈对灵芝多糖和灵芝酸含量的影响  

Effect of Respective Additions of Selenium and Cerium on Contents of Polysaccharide and Triterpene in Fermentation Supernatant and Mycelia of Ganoderma lucidum

作　　者：孙娟[1] 温鲁[1] 陈菊萍[1] 翁梁[1] 汪敬健[1] 冯瀚[1] 

机构地区：[1]淮阴师范学院资源微生物研究所,江苏淮安223300

出　　处：《食品科学》2010年第7期86-89,共4页Food Science

基　　金：江苏省大学生实践创新计划立项资助项目;淮阴师范学院教授基金项目(06HSJS027)

摘　　要：目的:考察在培养液中添加硒和铈对灵芝多糖和灵芝酸含量的影响。方法:向培养液中分别添加不同质量浓度的亚硒酸钠或硝酸铈铵,用液体浅层静置培养法培养灵芝菌丝体,用苯酚-硫酸法测定菌丝体和菌液中的多糖,用冰醋酸-香草醛法测定菌丝体中的灵芝酸。结果:添加40mg/L亚硒酸钠培养的灵芝菌丝体,多糖含量比对照提高42.3%,灵芝酸含量比对照提高10.6%;添加15mg/L硝酸铈铵培养的灵芝菌丝体,多糖含量比对照提高51.1%,灵芝酸含量则有所降低;两种元素还可明显提高菌液中灵芝多糖的含量。结论:向培养液中适量添加硒或铈,可显著提高灵芝菌丝体和菌液中的多糖含量,硒还可同时提高菌丝体的灵芝酸含量,铈则降低灵芝酸含量。生产中可根据实际需要添加硒或铈,以获得品质优良的灵芝菌丝体和菌液。Objective：To investigate the effect of respective additions of selenium and cerium at various concentration levels on contents of polysaccharides and triterpene in fermentation supernatant and mycelia of Ganoderma lucidum.Methods：Ganoderma lucidum was statically grown for 30 d in shallow liquid medium containing various concentrations of sodium selenite or ammonium ceric nitrate.At the end of fermentation,the fermentation broth was harvested and separated into supernatant and mycelia to measure polysaccharide content in both of them by phenol-sulfuric acid method and ganoderic acid content in the mycelia by glacial acetic acid-vanillina method.Results：Added sodium selenite at the level of 40 mg/L to culture medium resulted in 42.3% and 10.6% increases of polysaccharides and ganoderic acid contents in the mycelia,respectively.An increase of 51.1% in polysaccharide content in the mycelia resulting from the addition of 15 mg/L ammonium ceric nitrate was observed,whereas ganoderic acid content exhibited a decrease.Respective additions of selenium and cerium resulted in an obvious increase of polysaccharide content in the supernatant.Conclusion：Respective additions of selenium and cerium to culture medium at an appropriate level significantly enhance polysaccharide contents in m fermentation supernatant and mycelia of Ganoderma lucidum,although added selenium increases ganoderic acid content,while added cerium decreases ganoderic acid content.To obtain high-quality fermentation supernatant and mycelia of Ganoderma lucidum,selenium and cerium should be added appropriately according to actual production requirements.

关 键 词：灵芝 亚硒酸钠 硝酸铈铵 灵芝多糖 三萜类化合物 灵芝酸 

分 类 号：R284.2[医药卫生—中药学]