油茶籽粕蛋白提取工艺研究  

Orthogonal Array Design for Extraction Optimization of Protein from Camellia oleifera Seed Meal

作　　者：丁丹华[1] 彭光华 夏辉[1] 万辉[3] 何东平[3] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070 [2]西藏自治区质量技术监督局,西藏拉萨850000 [3]武汉工业学院食品科学与工程学院,湖北武汉430023

出　　处：《食品科学》2010年第8期102-105,共4页Food Science

摘　　要：对油茶籽粕的基本组分进行测定,采用碱溶酸沉法制备油茶籽粕蛋白,在测定等电点基础上探讨油茶籽粕粉碎粒度、浸提液pH值、料液比、浸提时间、浸提温度对蛋白提取率的影响。设计正交试验,得出提取油茶籽粕蛋白最佳工艺条件为料液比1:25(g/mL)、pH10、浸提时间80min、浸提温度45℃和粉碎粒度80目,在此条件下,蛋白提取率为57.8%。The basic chemical composition of Camellia oleifera seed meal and the isoelectric point of Camellia oleifera seed meal protein were determined.According to the determination results,a method based on alkaline extraction followed by acid precipitation was proposed for extraction of protein from Camellia oleifera seed meal.Single factor and orthogonal array design methods were employed to examine the effects of alkaline extraction parameters including material particle size,material/liquid ratio,pH and extraction time and temperature on protein yield.Results indicated that the extraction for 80 min at pH 10 and a material/liquid ratio of 1：25 gave a maximum protein yield of 57.8% from material powder with particle sizes below 80 mesh.

关 键 词：油茶籽粕 蛋白质 提取 

分 类 号：TQ93[化学工程]