高效液相色谱法同时测定食品中的12种抗氧化剂  

Simultaneous Determination of 12 Antioxidants in Foods by High-performance Liquid Chromatography

作　　者：蔡发[1] 段小娟[1] 牟志春[1] 王曼霞[1] 王岩[1] 徐琴[1] 

机构地区：[1]山东出入境检验检疫局,山东青岛266002

出　　处：《食品科学》2010年第8期207-211,共5页Food Science

基　　金：山东出入境检验检疫局科研项目(SK200889)

摘　　要：目的:建立一种快速、准确测定食品中12种抗氧化剂的高效液相色谱法。方法:样品用正己烷溶解,用含抗坏血酸棕榈酸盐(AP)的饱和乙腈萃取,以反相C18柱为分离柱,以甲醇-乙腈-乙酸-水体系为流动相进行梯度洗脱,采用高效液相色谱-紫外检测器于280nm定量检测。结果:12种抗氧化剂在36min内完全分离,线性范围为0.2～200mg/L(r=0.9981～0.9999),定量限为0.2～1.0mg/kg,回收率为81.13%～107.57%,相对标准偏差为0.26%～4.52%(n=7)。结论:本方法准确、可靠、简便、检出限低,适合分析大批量样品。This study aimed to present a high-performance liquid chromatographic（HPLC） method for the determination of 12 antioxidants in foods.Samples were dissolved in n-hexane saturated with acetonitrile and extracted with the lower phase（containing ascorbyl palmitate） resulting from n-hexane saturated with acetonitrile.A C18 column and a mobile phase consisting of methanol,acetonitrile,acetic acid and water were used for the chromatographic separation in the gradient elution mode.The detection wavelength of UV detector was set at 280 nm.Results showed that the thorough separation of 12 antioxidants was achieved within 36 min.The method exhibited limits of quantification（LOQ） ranging from 0.2 to 1.0 mg/kg and a linear range of 0.2 to 200 mg/L（r = 0.9981-0.9999）.Mean spike recoveries at 3 levels of 7 replicates for 12 antioxidants in 3 representative samples with complicated matrix such as palm oil,cake and instant noodles were within the range of 81.13%-107.57%,with a relative standard derivation range of 0.26%-4.52%.The method demonstrates the advantages of simplicity,reliability and low LOQ,and is consequently most suitable for large-batch analysis of antioxidants in foods.

关 键 词：抗氧化剂 高效液相色谱法(HPLC) 食品 

分 类 号：TS207.3[轻工技术与工程—食品科学]