2007-2008陕西部分零售畜禽肉沙门氏菌血清型和基因型  

Serotypic and genotypic characterization of Salmonella Serovars from retails meat in Shaanxi province (2007 2008)

作　　者：杨保伟[1] 张秀丽[2] 曲东[1] 席美丽[3] 崔生辉[4] 申进玲[3] 赵彪[3] 寄宝义[3] 孟江洪[3] 

机构地区：[1]西北农林科技大学生命科学学院,杨凌712100 [2]河南省疾病预防控制中心,郑州450016 [3]西北农林科技大学食品科学与工程学院,杨凌712100 [4]中国药品生物制品检定所,北京100050

出　　处：《微生物学报》2010年第5期654-660,共7页Acta Microbiologica Sinica

基　　金：长江学者讲座教授奖励计划项目(Z111020001);西北农林科技大学留学回国人员基金(Z111020525)~~

摘　　要：【目的】研究359株在2007-2008年分离于陕西部分地区零售畜禽肉中沙门氏菌的血清型和基因型,为确保食品安全提供依据。【方法】使用WHO指定的泰国S&A公司的沙门氏菌诊断血清,采用玻片凝集法测定沙门氏菌的血清型。使用美国疾病预防控制中心推荐的脉冲场凝胶电泳方法确定沙门氏菌的DNA酶切图谱,BioNumerics软件分析电泳结果,确定沙门氏菌的基因型。【结果】359株沙门氏菌共检出24个血清型,以肠炎沙门氏菌(Salmonella Enteritidis)、鼠伤寒沙门氏菌(S.Typhimurium)、舒卜拉沙门氏菌(S.Shubra)、印第安纳沙门氏菌(S.Indiana)和德尔卑沙门氏菌(S.Derby)等为主,不常见血清型有圣保罗沙门氏菌(Salmonella Saintpaul)、里地乌沙门氏菌(S.Rideau)、田纳西沙门氏菌(S.Tennessee)、汤卜逊沙门氏菌(S.Thompson)、加里玛沙门氏菌(S.Galiema)、卡罗尔沙门氏菌(S.Kallo)、沙门氏菌Ⅲa(S.Ⅲa)、罗森沙门氏菌(S.Rissen)和布兰卡斯特沙门氏菌(S.Brancaster)等。鸡肉中最常见血清型为肠炎沙门氏菌,猪肉和羊肉中以德尔卑沙门氏菌检出率最高,而牛肉中以里地乌沙门氏菌为主。359株沙门氏菌使用XbaⅠ酶切分型后,按照88%的基因同源性,可分为7个大簇。同一血清型的沙门氏菌分型后基本位于同一大簇之中,虽然部分沙门氏菌的血清型不同,但分型后仍表现出较高的基因同源性和相似的基因型。【结论】陕西零售肉沙门氏菌的血清型和基因型表现为多样性的特征。[Objective] Salmonella isolates isolated from retail meats in Shaanxi Province in 2007-2008 were characterized to determine their serotypes and genotypes.[Methods]Serotyping of 359 Salmonella was performed using slide agglutination method using Salmonella hyperimmune sera,whereas genomic DNA profiles were obtained using pulse field gel electrophoresis （PFGE） with XbaI.The data were analyzed and a dendrogram was generated using the BioNumerics Software.[Results]A total of 24 serotypes were identified among the 359 Salmonella isolates.The most common serotypes were Enteritidis,Typhimurium,Shubra,Indiana and Derby.Other serotypes were also identified,including Saintpaul,Rideau,Tennessee,Thompson,Galiema,Kallo,Ⅲ a,Rissen and Brancaster.Enteritidis and Rideau were most common in chicken and beef,respectively,whereas Derby was most common in in pork and lamb.Seven PFGE clusters were observed among the 359 isolates with 88% similarity.Salmonella isolates with the same serotypes were assigned to the same clusters with only a few exceptions.[Conclusion]Salmonella present in retail meats were phenotypically and genotypically diverse.A database on serotyping and molecular subtyping of Salmonella will enable us better understand the epidemiology of foodborne salmonellosis and identify their source more rapidly and accurately.

关 键 词：零售肉 沙门氏菌 血清型 基因型 

分 类 号：S851.43[农业科学—预防兽医学]