锯缘青蟹原肌球蛋白过敏者血清的筛选  

Screening of Serum in Patients Allergic to Tropomyosin of Mangrove Crab(Scylla serrata)

作　　者：李兵[1,2] 刘光明[1,2] 蔡秋凤[1,2] 黄园园[1,2] 苏文金[1,2] 曹敏杰[1,2] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2010年第3期185-189,共5页Journal of Jimei University：Natural Science

基　　金：国家自然科学基金资助项目(30871947);福建省自然科学基金资助项目(2008J0067);福建省高校水产科学技术与食品安全重点实验室开放基金资助项目(2007J402);集美大学中青年创新团队基金资助项目(2008A002);集美大学科研启动基金资助项目(F05039)

摘　　要：为了解集美大学学生的饮食及食物过敏情况,对在校学生进行了问卷调查.调查共分发了4 000份,主要涉及水产品食物过敏的种类、过敏症状等.调查收到有效问卷3 890份,应答率为97.3%.自诉过敏的学生为1 149人,约占29.5%.对水产品食物过敏的有545人(占自诉过敏的47.4%),其中31例经ELISA检测,IgE为对蟹的原肌球蛋白呈阳性反应(占水产品食物过敏的5.7%,占蟹过敏的11.3%).男女之间无统计学差异.自诉对蟹、虾、贝、鱼等水产品的过敏症状相似,多为皮疹和皮肤瘙痒.调查结果表明,蟹、虾、贝类和鱼是主要的水产品过敏食物.食物过敏症患者血清食物过敏原特异性IgE升高明显,采用ELISA检测法,方便,安全,准确,对临床确诊与防治食物过敏症有重要意义.In order to get epidemiologic data of food allergy of Jimei University students and supply some references for the study of food allergy,4 000 questionnaires to the students were distributed .In addition,the relationship between allergenic foods and symptoms was analyzed.3 890（97.3 %） effective questionnaire responses were received.1 149 students（29.5 %） had suffered food allergy once at least,and 545 of them（about 47.4 %） were to aquatic products.31 students（about 5.7 % of those who suffered aquatic product allergy and 11.3 % of those who suffered crab allergy） showed a positive reaction to the crab by the ELISA test.There was no statistic difference in incidence between sexes.The students allergic to crab,shrimp,seashell and fish had similar symptoms,mostly as rash and skin-tickle.This survey showed that crab,shrimp,seashell and fish were the major allergenic food of aquatic products.Food specific IgE levels were significantly increased in allergic patients.The detection was convenient,safe and accurate by use of ELISA,suggesting that the measurement of food specific IgE in patients with allergy might be useful for its diagnosis,prevention and treatment.

关 键 词：食物过敏 水产品 问卷调查 ELISA 

分 类 号：TS207[轻工技术与工程—食品科学]