4株益生菌的表面特性及抑制致病菌作用研究  

Study on Cell Surface Properties and Inhibitory Effects on Pathogens of Four Probiotic Strains

作　　者：刘勇[1] 张勇[1] 包艳[1] 张和平[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,国家奶牛产业技术研发中心乳制品加工研究室,内蒙古呼和浩特010018

出　　处：《中国食品学报》2010年第2期28-34,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家高技术研究发展计划(863计划;No.2006AA10Z345;2007AA10Z353);现代农业产业技术体系建设专项资金资助

摘　　要：为探索细胞表面特性与抗菌能力之间的关系,对干酪乳杆菌Zhang(Lactobacillus casei Zhang)、动物双歧杆菌V9(Bifidobacteria.animalis V9)、动物双歧杆菌Bb12(Bifidobacteria.animalis subsp.lactic Bb12)及干酪乳杆菌Shirota(Lactobacillus casei Shirota)的菌体表面特性(菌体凝集特性、表面疏水特性)及对肠道致病菌的抑制特性进行了研究,结果显示:各菌株自凝集能力随培养时间的延长均呈上升趋势;对致病菌的凝集率随益生菌、致病菌和培养时间的变化而不同;具有较高自凝集能力的菌株,其疏水能力及对致病菌的凝集能力同样较高;各益生菌对致病菌的凝集能力与其产生抑菌物质的抑菌能力无明显关系。In order to investigate the relationship between cell surface properties and antibacterial activities,four probiotics（Lactobacillus casei Zhang,Bifidobacterium animalis V9,Bifidobacterium animalis subsp.lactic Bb12 and Lactobacillus casei Shirota） were evaluated for the ability to autoaggregate,together with cell-surface hydrophobicity and coaggregation and inhibitory abilities with pathogen strains in vitro.The autoaggregation abilities of the tested four strains were increased with time during the incubation.All tested four strains showed the abilities of coaggregation with pathogens which were strain-specific and independent on probiotic,pathogen and incubative time.High levels of coaggregation with pathogens were comparable to high autoaggregating properties,and high autoaggregating properties were relevant to high hydrophobicity of cell surface.No obvious relevance was found between coaggregation with pathogens and inhibitory effect of the antibacterial substances of probiotics since two different mechanisms involved.

关 键 词：益生菌 凝集特性 细胞表面疏水特性 抑菌作用 

分 类 号：R371[医药卫生—病原生物学]